Hemostatic patch with ultra-strengthened mechanical properties for efficient adhesion to wet surfaces

被引:49
作者
Zheng, Yuting [1 ]
Shariati, Kaavian [2 ]
Ghovvati, Mahsa [1 ,3 ]
Vo, Steven [1 ]
Origer, Nolan [1 ]
Imahori, Taichiro [3 ]
Kaneko, Naoki [3 ]
Annabi, Nasim [1 ,4 ]
机构
[1] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
[2] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA
[3] Univ Calif Los Angeles, David Geffen Sch Med, Dept Radiol Sci, Div Intervent Neuroradiol, Los Angeles, CA 90095 USA
[4] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA
基金
美国国家卫生研究院;
关键词
Molecular design; Tough hydrogels; Bioadhesives; Hemostatic materials; TISSUE ADHESIVENESS; INSPIRED ADHESIVE; TOUGH ADHESION; CROSS-LINKS; HYDROGEL; ANTIBACTERIAL; ALGINATE; BIOADHESIVE; ELASTICITY; STRATEGIES;
D O I
10.1016/j.biomaterials.2023.122240
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Controlling traumatic bleeding from damaged internal organs while effectively sealing the wound is critical for saving the lives of patients. Existing bioadhesives suffer from blood incompatibility, insufficient adhesion to wet surfaces, weak mechanical properties, and complex application procedures. Here, we engineered a ready-to-use hemostatic bioadhesive with ultra-strengthened mechanical properties and fatigue resistance, robust adhesion to wet tissues within a few seconds of gentle pressing, deformability to accommodate physiological function and action, and the ability to stop bleeding efficiently. The engineered hydrogel, which demonstrated high elasticity (>900%) and toughness (>4600 kJ/m3), was formed by fine-tuning a series of molecular interactions and crosslinking mechanisms involving N-hydroxysuccinimide (NHS) conjugated alginate (Alg-NHS), poly (ethylene glycol) diacrylate (PEGDA), tannic acid (TA), and Fe3+ ions. Dual adhesive moieties including mussel-inspired pyrogallol/catechol and NHS synergistically enhanced wet tissue adhesion (>400 kPa in a wound closure test). In conjunction with physical sealing, the high affinity of TA/Fe3+ for blood could further augment he-mostasis. The engineered bioadhesive demonstrated excellent in vitro and in vivo biocompatibility as well as improved hemostatic efficacy as compared to commercial Surgicel & REG;. Overall, the hydrogel design strategy described herein holds great promise for overcoming existing obstacles impeding clinical translation of engi-neered hemostatic bioadhesives.
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页数:18
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