Toxoplasma gondii molecular detection and phylogenetic analysis in hemodialysis patients from Khuzestan, Southwest Iran

被引：2

作者：

Yarahmadi, Saba ^{[1
]}

Saki, Jasem ^{[2
]}

Arjmand, Reza ^{[1
]}

Shahbazian, Heshmatolah ^{[3
]}

机构：

[1] Ahvaz Jundishapur Univ Med Sci, Sch Med, Dept Med Parasitol, POB 6135743337, Ahvaz 39361155, Iran

[2] Ahvaz Jundishapur Univ Med Sci, Hlth Res Inst, Infect & Trop Dis Res Ctr, POB 6135743337, Ahvaz 39361155, Iran

[3] Ahvaz Jundishapur Univ Med Sci, Golestan Gen Hosp, Diabet & CKD Res Ctr, Dept Nephrol, POB 6135743337, Ahvaz 39361155, Iran

来源：

TROPICAL MEDICINE AND HEALTH | 2024年 / 52卷 / 01期

关键词：

Hemodialysis; Nested-PCR; Sequence; Toxoplasma gondii; STAGE RENAL-DISEASE; INFECTION; PREVALENCE;

D O I：

10.1186/s41182-024-00585-5

中图分类号：

R188.11 [热带医学];

学科分类号：

摘要：

Background The diagnosis and genetic characterization of Toxoplasma gondii (T. gondii) infection can make a significant influence to the prevention of the dangerous consequences of toxoplasmosis, particularly in immunocompromised people. Objective The aim of this investigation was to assess the frequency and genotyping of T. gondii in blood samples of patients with hemodialysis. Materials and methods In the current investigation, a total of 379 blood samples were taken from subjects with hemodialysis who were referred to teaching hospital of Ahvaz in the southwest of Iran. The samples were evaluated using the Nested PCR by targeting the B1 gene, and then, sequencing and phylogenetic tree were constructed. Results T. gondii DNA was found in 112 (29.55%) of the blood samples by Nested PCR. Amplicons from T. gondii revealed high identity with GenBank sequences. The phylogenetic analysis revealed that all sequences were closely related to Type I of T. gondii. Conclusion Because of the high incidence of toxoplasmosis with type I prevalent in hemodialysis patients, we recommend a systematic screening for toxoplasmosis to carry out for monitoring the possible dissemination of toxoplasmosis during hemodialysis.

引用

页数：7

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