A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella

被引：0
作者：
Chang, Jiang [1 ]
Wang, Nan [2 ]
Zhan, Jun-Peng [1 ,3 ]
Zhang, Shi-Jun [1 ]
Zou, De-Ying [1 ,4 ]
Li, Feng [1 ,5 ]
Zhang, Ying [1 ]
Li, Yan-Song [1 ]
Hu, Pan [1 ]
Lu, Shi-Ying [1 ]
Liu, Zeng-Shan [1 ]
Ren, Hong-Lin [1 ]
机构：
[1] Jilin Univ, Coll Vet Med, State Key Lab Diag & Treatment Severe Zoonot Infec, Key Lab Zoonosis Res,Minist Educ,Inst Zoonosis,Col, Changchun, Peoples R China
[2] Jilin Acad Anim Husb & Vet Med, Changchun, Peoples R China
[3] Dalian Univ, Coll Med, Dalian, Peoples R China
[4] Panjin Ctr Inspect & Testing, Panjin 124000, Peoples R China
[5] Shandong Binzhou Anim Sci & Vet Med Inst, Binzhou, Peoples R China
来源：
FOLIA MICROBIOLOGICA | 2024年 / 69卷 / 04期
关键词：
Brucella; BCSP31; gene; Recombinase polymerase amplification; SYBR Green I; Visualization; BACTERIAL COMMUNITY STRUCTURE; RIBOSOMAL-RNA; PANAX-NOTOGINSENG; RHIZOSPHERE SOIL; ROOT-ROT; DIVERSITY; QUALITY;
D O I：
10.1007/s12223-023-01115-2
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Brucellosis is a zoonosis caused by Brucella, which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important measure to prevent brucellosis, but the traditional method is time-consuming and not suitable for field applications. In this study, a recombinase polymerase amplification-SYBR Green I (RPAS) assay was developed for the rapid and visualized detection of Brucella in the field by targeting BCSP31 gene, a conserved marker. The method was highly specific without any cross-reactivity with other common bacteria and its detection limit was 2.14 x 10(4) CFU/mL or g of Brucella at 40 degree celsius for 20 min. It obviates the need for costly instrumentation and exhibits robustness towards background interference in serum, meat, and milk samples. In summary, the RPAS assay is a rapid, visually intuitive, and user-friendly detection that is highly suitable for use in resource-limited settings. Its simplicity and ease of use enable swift on-site detection of Brucella, thereby facilitating timely implementation of preventive measures.
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收藏
页码：767 / 774
页数：8
相关论文
共 23 条
  • [1] Interactions of the Human Pathogenic Brucella Species with Their Hosts
    Atluri, Vidya L.
    Xavier, Mariana N.
    de Jong, Maarten F.
    den Hartigh, Andreas B.
    Tsolis, Renee M.
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, VOL 65, 2011, 65 : 523 - 541
  • [2] Bovine Brucellosis in Argentina and Bordering Countries: Update
    Aznar, M. N.
    Samartino, L. E.
    Humblet, M. -F.
    Saegerman, C.
    [J]. TRANSBOUNDARY AND EMERGING DISEASES, 2014, 61 (02) : 121 - 133
  • [3] BAILY GG, 1992, J TROP MED HYG, V95, P271
  • [4] Fluorescent cyanine dyes for the quantification of low amounts of dsDNA
    Bruijns, B. B.
    Tiggelaar, R. M.
    Gardeniers, J. G. E.
    [J]. ANALYTICAL BIOCHEMISTRY, 2016, 511 : 74 - 79
  • [5] Molecular epidemiological characterization of Brucella isolates from sheep and yaks in northwest China
    Cao, X.
    Li, Z.
    Liu, Z.
    Fu, B.
    Liu, Y.
    Shang, Y.
    Zhou, J.
    Jing, Z.
    [J]. TRANSBOUNDARY AND EMERGING DISEASES, 2018, 65 (02) : e425 - e433
  • [6] Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I
    Chen, Xiuqin
    Zhao, Lianjing
    Wang, Jiahui
    Wang, Haolu
    Qiu, Yangyuan
    Dong, Zijian
    Zhang, Chunling
    Liu, Mingyuan
    Wang, Xuelin
    Bai, Xue
    [J]. FRONTIERS IN MICROBIOLOGY, 2022, 13
  • [7] Improvement and Advancement of Early Diagnosis of Human Brucellosis in Window Period
    Chen, Zeliang
    Wang, Yufei
    Wang, Zhoujia
    Ke, Yuehua
    Zhen, Qing
    Yuan, Xitong
    Zhang, Wenyi
    Lu, Yong
    Yu, Yaqing
    Song, Hongbin
    Huang, Liuyu
    [J]. CLINICAL INFECTIOUS DISEASES, 2013, 57 (02) : 322 - +
  • [8] Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review
    Chlebicz, Agnieszka
    Slizewska, Katarzyna
    [J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 2018, 15 (05)
  • [9] Recombinase Polymerase Amplification for Diagnostic Applications
    Daher, Rana K.
    Stewart, Gale
    Boissinot, Maurice
    Bergeron, Michel G.
    [J]. CLINICAL CHEMISTRY, 2016, 62 (07) : 947 - 958
  • [10] Bayesian Evaluation of Three Serological Tests for Diagnosis of Brucella infections in Dromedary Camels Using Latent Class Models
    Elsohaby, Ibrahim
    Kostoulas, Polychronis
    Elsayed, Ahmed M.
    Ahmed, Heba A.
    El-Diasty, Mohamed M.
    Wareth, Gamal
    Ghanem, Fatma M.
    Arango-Sabogal, Juan Carlos
    [J]. PREVENTIVE VETERINARY MEDICINE, 2022, 208
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