Accessing the intrinsic factors of carbonyl index of microplastics: Physical and spectral properties, baseline correction, calculation methods, and their interdependence

Carbonyl index (CI) is a measure of the degradation status of microplastics. While many studies address environmental factors of microplastic degradation, intrinsic factors like physical properties, spectral properties, baseline correction, and CI calculation methods are less explored. This research focused on these aspects using surface seawater samples. We found that color and shape have limited dependence on particle size or signal-tonoise ratio (SNR). Baseline correction can significantly alter CI values, with the direction of the shift depending on the methods used. Additionally, most CI values before and after baseline correction and those calculated using different methods tend to be strongly correlated. Using the selected CI calculation methods, we found that CI values varied significantly by shape and color. CI's relation to the similarity between the sample and its pristine form suggests an alternative degradation measure. Our findings emphasize the need for standardized CI calculation methods.