Side-effects of a number of insecticides on predatory mites in apple orchards

Background: Amblyseius andersoni is a common predatory mite occurring in fruit orchards located in Europe and North America. Its role in preventing spider mite outbreaks is widely recognized, in particular when selective pesticides are used. The compatibility between plant protection products and predatory mites is crucial to preserve their activity. There is a need to investigate the effects of pesticides on beneficials using multiple approaches. Objectives: Field and laboratory experiments were conducted to evaluate the effects of a number of insecticides on A. andersoni. Methods: The effects of neonicotinoids (i.e., acetamiprid, imidacloprid, thiacloprid, thia-methoxam) were compared with those of pyrethroids (i.e., tau-fluvalinate), well known for their negative impact on predatory mites. Insecticides were applied 1-3 times in an experimental fruit orchard located in Northern Italy. Laboratory trials focused on their effects on the survival and the fecundity of predatory mite females. Results: Field experiments showed a decline in predatory mite numbers in plots treated with neonicotinoids or tau-fluvalinate compared to the untreated control. However, predatory mites in neonicotinoid plots reached higher densities compared to those recorded in tau-fluvalinate plots. Spider mite (Panonychus ulmi) populations reached moderate to high densities in plots treated with tau-fluvalinate while their densities were negligible in the remaining plots. Amblyseius andersoni survival was moderately affected by some neonicotinoids in the laboratory while they significantly reduced predatory mite fecundity. In contrast tau-fluvalinate exerted severe effects on survival and fecundity of predatory mites. Finally, escaping rate increased after pesticide exposure suggesting possible alterations in predatory mite behavior. Conclusions: Neonicotinoid applications significantly affected predatory mite densities in field conditions and this phenomenon appeared to be influenced by their impact on female fecundity. Their effects on survival were less severe. Implications of these results for IPM tactics in fruit orchards are discussed.