Site-Specific Profiling of N-Glycans in Drosophila melanogaster

被引:1
|
作者
Zhao, Fei [1 ]
Jia, Chenyu [1 ]
He, Fangyu [1 ]
Hu, Meiting [1 ]
Guo, Xingyu [1 ]
Zhang, Jiaxin [1 ]
Feng, Xuesong [1 ]
机构
[1] Shaanxi Univ Chinese Med, Coll Basic Med Sci, Xianyang 712046, Shaanxi, Peoples R China
来源
FRONTIERS IN BIOSCIENCE-LANDMARK | 2023年 / 28卷 / 11期
关键词
Drosophila; N; -glycans; intact glycopeptide analysis; LC-MS/MS; glyco-related enzymes; PROTEIN GLYCOSYLATION; LINKED GLYCOPROTEINS; DIVERSITY;
D O I
10.31083/j.fbl2811278
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Background: Drosophila melanogaster is a well-studied and highly tractable genetic model system for deciphering the molecular mechanisms underlying various biological processes. Although being one of the most critical post-translational modifications of proteins, the understanding of glycosylation in Drosophila is still lagging behind compared with that of other model organisms. Methods: In this study, we systematically investigated the site-specific N-glycan profile of Drosophila melanogaster using intact glycopeptide analysis technique. This approach identified the glycans, proteins, and their glycosites in Drosophila, as well as information on site-specific glycosylation, which allowed us to know which glycans are attached to which glycosylation sites. Results: The results showed that the majority of N-glycans in Drosophila were high-mannose type (69.3%), consistent with reports in other insects. Meanwhile, fucosylated N-glycans were also highly abundant (22.7%), and the majority of them were mono-fucosylated. In addition, 24 different sialylated glycans attached with 16 glycoproteins were identified, and these proteins were mainly associated with developmental processes. Gene ontology analysis showed that N-glycosylated proteins in Drosophila were involved in multiple biological processes, such as axon guidance, N-linked glycosylation, cell migration, cell spreading, and tissue development. Interestingly, we found that seven glycosyltransferases and four glycosidases were N-glycosylated, which suggested that N-glycans may play a regulatory role in the synthesis and degradation of N-glycans and glycoproteins. Conclusions: To our knowledge, this work represents the first comprehensive analysis of site-specific N-glycosylation in Drosophila, thereby providing new perspectives for the understanding of biological functions of glycosylation in insects.
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页数:10
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