Cadmium induces chondroitin sulfate synthase 1 via protein kinase Ca and elongates chondroitin/dermatan sulfate chains in cultured vascular endothelial cells

被引：0

作者：

Hara, Takato ^{[1
]}

Matsuura, Shogo ^{[1
]}

Aikawa, Keith ^{[1
]}

Shirai, Misaki ^{[1
]}

Yoshida, Mai ^{[1
]}

Kaji, Toshiyuki ^{[2
]}

Yamamoto, Chika ^{[1
]}

机构：

[1] Toho Univ, Fac Pharmaceut Sci, Dept Environm Hlth, Funabashi, Chiba 2748510, Japan

[2] Tokyo Univ Sci, Fac Pharmaceut Sci, Dept Environm Hlth, Noda, Chiba 2788510, Japan

来源：

JOURNAL OF TOXICOLOGICAL SCIENCES | 2023年 / 48卷 / 08期

关键词：

Vascular endothelial cell; Cadmium; Proteoglycan; Chondroitin sulfate synthase 1; Chondroitin/dermatan sulfate; MOLECULAR-CLONING; SMOOTH-MUSCLE; DERMATAN SULFATE; N-ACETYLGALACTOSAMINYLTRANSFERASE; PROTEOGLYCANS; EXPRESSION; BINDING; WEIGHT; GLYCOSAMINOGLYCANS; POLYMERIZATION;

D O I：

暂无

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

Cadmium is an environmental pollutant and a risk factor for atherosclerosis. In the atherosclerotic intima, dermatan sulfate chains accelerate accumulation and oxidation of LDL cholesterol. The major type of dermatan sulfate proteoglycan that is synthesized by vascular endothelial cells is bigly-can. In the present study, we analyzed the effect of cadmium on the biglycan synthesis using cultured bovine aortic endothelial cells. Cadmium did not induce biglycan mRNA and core protein expression; however, it elongated the chondroitin/dermatan sulfate chains of biglycan. Among elongation enzymes of the chondroitin/dermatan sulfate chain, chondroitin sulfate synthase 1 (CHSY1) mRNA and protein expression were dose-and time-dependently upregulated by cadmium depending on protein kinase Ca. This finding suggests that CHSY1-dependent elongation of chondroitin/dermatan sulfate chains of bigly-can may exacerbate cadmium-induced atherosclerosis.

引用

页码：457 / 467

页数：11

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