Prolonging somatic cell proliferation through constitutive hox gene expression in C. elegans

被引:1
作者
Heinze, Svenia D. [1 ,2 ]
Berger, Simon [1 ,3 ]
Engleitner, Stefanie [1 ,2 ]
Daube, Michael [1 ]
Hajnal, Alex [1 ]
机构
[1] Univ Zurich, Dept Mol Life Sci, Winterthurerstr 190, CH-8057 Zurich, Switzerland
[2] Univ & ETH Zurich, Mol Life Sci PhD Program, CH-8057 Zurich, Switzerland
[3] Swiss Fed Inst Technol, Inst Chem and Bioengn, Vladimir Prelog Weg 1, CH-8093 Zurich, Switzerland
基金
瑞士国家科学基金会;
关键词
ANCHOR CELL; VULVAL DEVELOPMENT; STEM-CELLS; DEGRADATION; PROTEIN; GENOME; LINEAGES; INSIGHTS; NEMATODE; PATTERNS;
D O I
10.1038/s41467-023-42644-1
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
hox genes encode a conserved family of homeodomain transcription factors that are essential to determine the identity of body segments during embryogenesis and maintain adult somatic stem cells competent to regenerate organs. In contrast to higher organisms, somatic cells in C. elegans irreversibly exit the cell cycle after completing their cell lineage and the adult soma cannot regenerate. Here, we show that hox gene expression levels in C. elegans determine the temporal competence of somatic cells to proliferate. Down-regulation of the central hox gene lin-39 in dividing vulval cells results in their premature cell cycle exit, whereas constitutive lin-39 expression causes precocious Pn.p cell and sex myoblast divisions and prolongs the proliferative phase of the vulval cells past their normal point of arrest. Furthermore, ectopic expression of hox genes in the quiescent anchor cell re-activates the cell cycle and induces proliferation until young adulthood. Thus, constitutive expression of a single hox transcription factor is sufficient to prolong somatic cell proliferation beyond the restriction imposed by the cell lineage. The down-regulation of hox gene expression in most somatic cells at the end of larval development may be one cause for the absence of cell proliferation in adult C. elegans.
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页数:13
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