miR-210 Expression Is Strongly Hypoxia-Induced in Anaplastic Thyroid Cancer Cell Lines and Is Associated with Extracellular Vesicles and Argonaute-2

被引:7
作者
Powell, Bonita H. [1 ]
Turchinovich, Andrey [2 ,3 ]
Wang, Yongchun [4 ]
Gololobova, Olesia [1 ]
Buschmann, Dominik [1 ]
Zeiger, Martha A. [5 ]
Umbricht, Christopher B. [4 ]
Witwer, Kenneth W. [1 ,6 ]
机构
[1] Johns Hopkins Univ, Sch Med, Dept Mol & Comparat Pathobiol, Baltimore, MD 21205 USA
[2] German Canc Res Ctr, Div Canc Genome Res, D-69120 Heidelberg, Germany
[3] Heidelberg Biolabs GmbH, D-69120 Heidelberg, Germany
[4] Johns Hopkins Univ, Sch Med, Dept Surg, Baltimore, MD 21205 USA
[5] NCI, Ctr Canc Res, NIH, Bethesda, MD 20892 USA
[6] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA
关键词
microRNA; miRNA; miR-210; hypoxia; HIF1-alpha; extracellular vesicles; anaplastic thyroid cancer; INDUCIBLE FACTOR-I; MICRORNA EXPRESSION; CIRCULATING MICRORNA; UNITED-STATES; CARCINOMA; GENE; FACTOR-1-ALPHA; BIOMARKERS; PROTEIN; TARGET;
D O I
10.3390/ijms24054507
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hypoxia, or low oxygen tension, is frequently found in highly proliferative solid tumors such as anaplastic thyroid carcinoma (ATC) and is believed to promote resistance to chemotherapy and radiation. Identifying hypoxic cells for targeted therapy may thus be an effective approach to treating aggressive cancers. Here, we explore the potential of the well-known hypoxia-responsive microRNA (miRNA) miR-210-3p as a cellular and extracellular biological marker of hypoxia. We compare miRNA expression across several ATC and papillary thyroid cancer (PTC) cell lines. In the ATC cell line SW1736, miR-210-3p expression levels indicate hypoxia during exposure to low oxygen conditions (2% O-2). Furthermore, when released by SW1736 cells into the extracellular space, miR-210-3p is associated with RNA carriers such as extracellular vesicles (EVs) and Argonaute-2 (AGO2), making it a potential extracellular marker for hypoxia.
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页数:14
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