Antifungal susceptibility of the clinical and environmental strains of Cryptococcus gattii sensu lato in Taiwan

被引：1

作者：

Lin, Kuo-Hsi ^{[1
,2
]}

Lai, Yi-Chyi ^{[3
]}

Lin, Yi-Pei ^{[1
]}

Ho, Mao-Wang ^{[4
]}

Chen, Yee-Chun ^{[5
]}

Chung, Wen-Hsin ^{[2
,6
]}

机构：

[1] TungsTaichung MetroHarbor Hosp, Taichung, Taiwan

[2] Natl Chung Hsing Univ, Taichung, Taiwan

[3] Chung Shan Med Univ, Taichung, Taiwan

[4] China Med Univ Hosp, Taichung, Taiwan

[5] Natl Taiwan Univ Hosp, Taipei, Taiwan

[6] Innovat & Dev Ctr Sustainable Agr IDCSA, Taichung, Taiwan

来源：

MYCOSES | 2023年 / 66卷 / 01期

关键词：

amphotericin B; antifungal susceptibility profile; cryptococcosis; fluconazole; minimum inhibitory concentrations; sequence type; EPIDEMIOLOGIC CUTOFF VALUES; IN-VITRO SUSCEPTIBILITIES; END-POINT DISTRIBUTIONS; AMPHOTERICIN-B; PACIFIC-NORTHWEST; SPECIES COMPLEX; VARIETIES; NEOFORMANS; FLUCONAZOLE; RESISTANCE;

D O I：

10.1111/myc.13520

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

Background The rare occurrence of human cryptococcosis caused by Cryptococcus gattii sensu lato leads to difficulties in establishing the antifungal susceptibility profile between species of this potentially lethal pathogen, which may be crucial for treating cryptococcosis. Objective To establish an antifungal susceptibility profile of C. gattii s.l. in Taiwan. Methods A total of 104 environmental C. gattii s.l. strains (including multilocal sequence typing ST7, ST106, ST274, ST328, ST546, ST548 and ST630) and 21 previously collected clinical strains (including ST7, ST44, ST06, ST274, ST328 and ST329) were included in this study. We determined the minimum inhibitory concentrations (MICs) of six antifungal agents (itraconazole, fluconazole, voriconazole, posaconazole, flucytosine and amphotericin B) against environmental C. gattii s.l. strains and compared the antifungal susceptibility profiles of environmental strains with those of clinical strains. Results The antifungal susceptibility data demonstrated that the MICs of antifungal agents against environmental strains were comparable to those against clinical strains. Compared with strains of Cryptococcus deuterogattii, those of C. gattii sensu stricto were more susceptible to azoles and flucytosine. The differences in antifungal susceptibility between the strains of each sequence type (ST) were significant. Correlation analysis of MICs revealed cross-resistance between azoles in environmental strains of C. gattii s.l. Geographic differences in the antifungal susceptibility of C. gattii s.l. isolated from different cities in Taiwan were observed in this study. Conclusion Clinical and environmental strains were indistinguishable in antifungal susceptibility. The antifungal susceptibility of C. gattii s.l. is associated with STs. Therefore, establishing an ST-oriented domestic antifungal susceptibility database may help treat C. gattii s.l.-induced cryptococcosis.

引用

页码：13 / 24

页数：12

共 52 条

[1] Correlation of fluconazole MICs with clinical outcome in Cryptococcal infection [J].

Aller, AI ;

Martin-Mazuelos, E ;

Lozano, F ;

Gomez-Mateos, J ;

Steele-Moore, L ;

Holloway, WJ ;

Gutiérrez, MJ ;

Recio, FJ ;

Espinel-Ingroff, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2000, 44 (06) :1544-1548

[2] Anticytokine autoantibodies leading to infection: early recognition, diagnosis and treatment options [J].

Barcenas-Morales, Gabriela ;

Cortes-Acevedo, Pauline ;

Doffinger, Rainer .

CURRENT OPINION IN INFECTIOUS DISEASES, 2019, 32 (04) :330-336

[3] From the environment to the host: How non-azole agrochemical exposure affects the antifungal susceptibility and virulence of Cryptococcus gattii [J].

Bastos, Rafael Wesley ;

Cota Freitas, Gustavo Jose ;

Neves Oliveira, Hellem Cristina Silva Carneiro Lorena Vivien ;

Neves Oliveira, Lorena Vivien ;

Gouveia-Eufrasio, Ludmila ;

Nonato Santos, Anderson Philip ;

Moyrand, Frederique ;

Maufrais, Corinne ;

Janbon, Guilhem ;

Santos, Daniel Assis .

SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 681 :516-523

[4] Environmental Triazole Induces Cross-Resistance to Clinical Drugs and Affects Morphophysiology and Virulence of Cryptococcus gattii and C. neoformans [J].

Bastos, Rafael Wesley ;

Silva Carneiro, Hellem Cristina ;

Neves Oliveira, Lorena Vivien ;

Rocha, Karen Maia ;

Cota Freitas, Gustavo Jose ;

Costa, Marliete Carvalho ;

Ferreira Magalhaes, Thais Furtado ;

Dutra Carvalho, Vanessa Silva ;

Rocha, Claudia Emanuela ;

Ferreira, Gabriella Freitas ;

Paixao, Tatiane Alves ;

Moyrand, Frederique ;

Janbon, Guilhem ;

Santos, Daniel Assis .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2018, 62 (01)

[5] Multilocus sequence typing of strains from the Cryptococcus gattii species complex from different continents [J].

Bellet, Virginie ;

Roger, Frederic ;

Krasteva, Donika ;

Gouveia, Tiphany ;

Drakulovski, Pascal ;

Pottier, Cyril ;

Bertout, Sebastien .

MYCOSES, 2022, 65 (01) :88-96

[6] AZOLE DRUG-RESISTANCE AS A CAUSE OF CLINICAL RELAPSE IN AIDS PATIENTS WITH CRYPTOCOCCAL MENINGITIS [J].

BIRLEY, HDL ;

JOHNSON, EM ;

MCDONALD, P ;

PARRY, C ;

CAREY, PB ;

WARNOCK, DW .

INTERNATIONAL JOURNAL OF STD & AIDS, 1995, 6 (05) :353-355

[7] SUSCEPTIBILITIES OF SERIAL CRYPTOCOCCUS-NEOFORMANS ISOLATES FROM PATIENTS WITH RECURRENT CRYPTOCOCCAL MENINGITIS TO AMPHOTERICIN-B AND FLUCONAZOLE [J].

CASADEVALL, A ;

SPITZER, ED ;

WEBB, D ;

RINALDI, MG .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (06) :1383-1386

[8] Antifungal Therapy and Management of Complications of Cryptococcosis due to Cryptococcus gattii [J].

Chen, Sharon C. -A. ;

Korman, Tony M. ;

Slavin, Monica A. ;

Marriott, Deborah ;

Byth, Karen ;

Bak, Narin ;

Currie, Bart J. ;

Hajkowicz, Krispin ;

Heath, Christopher H. ;

Kidd, Sarah ;

McBride, William J. H. ;

Meyer, Wieland ;

Murray, Ronan ;

Playford, E. Geoffrey ;

Sorrell, Tania C. .

CLINICAL INFECTIOUS DISEASES, 2013, 57 (04) :543-551

[9] Clinical features and in vitro susceptibilities of two varieties of Cryptococcus neoformans in Taiwan [J].

Chen, YC ;

Chang, SC ;

Shih, CC ;

Hung, CC ;

Luh, KT ;

Pan, YS ;

Hsieh, WC .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2000, 36 (03) :175-183

[10] In vitro antifungal susceptibility profiles and genotypes of 308 clinical and environmental isolates of Cryptococcus neoformans var. grubii and Cryptococcus gattii serotype B from north-western India [J].

Chowdhary, Anuradha ;

Randhawa, Harbans Singh ;

Sundar, Gandhi ;

Kathuria, Shallu ;

Prakash, Anupam ;

Khan, Ziauddin ;

Sun, Sheng ;

Xu, Jianping .

JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 60 (07) :961-967

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