The effect of insulin resistance on extracellular BCAA accumulation and SLC25A44 expression in a myotube model of skeletal muscle insulin resistance

被引：3
作者：
Rivera, Caroline N. [1 ]
Watne, Rachel M. [2 ]
Wommack, Andrew J. [2 ]
Vaughan, Roger A. [1 ]
机构：
[1] High Point Univ, Dept Hlth & Human Performance, One Univ Pkwy, High Point, NC 27262 USA
[2] High Point Univ, Dept Chem, High Point, NC USA
来源：
AMINO ACIDS | 2023年 / 55卷 / 11期
关键词：
Leucine; Isoleucine; Valine; pAkt; Akt; Insulin resistance; Diabetes; CHAIN AMINO-ACIDS;
D O I：
10.1007/s00726-023-03336-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Insulin resistance is often accompanied by elevated circulating branched-chain amino acids (BCAA). We investigated the effects of insulin resistance on the mitochondrial BCAA transporter, SLC25A44, using a myotube model of insulin resistance. Insulin sensitivity and SLC25A44 expression were assessed via Western blot. Liquid chromatography-mass spectrometry was used to evaluate extracellular BCAA media content. Insulin resistance reduced pAkt activation following insulin stimulation but did not alter SLC25A44 expression. Under select conditions, insulin resistance led to the accumulation of extracellular BCAA.
引用
收藏
页码：1701 / 1705
页数：5
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