Expression patterns of Piezo1 in the developing mouse forebrain

被引:1
|
作者
Kim, Hye Yoon [1 ,2 ]
Kang, Bokeum [1 ]
Lee, Pa Reum [1 ]
Kim, Kyungmin [1 ]
Hong, Gyu-Sang [1 ,2 ,3 ]
机构
[1] Korea Inst Sci & Technol KIST, Brain Sci Inst, Seoul, South Korea
[2] Univ Sci & Technol, KIST Sch, Div Biomed Sci & Technol, Seoul, South Korea
[3] Seoul Natl Univ, Grad Sch Convergence Sci & Technol, Dept Mol Med & Biopharmaceut Sci, Seoul, South Korea
来源
BRAIN STRUCTURE & FUNCTION | 2024年 / 229卷 / 03期
基金
新加坡国家研究基金会;
关键词
Piezo1; Mechanical cues; Brain development; Embryogenesis; Neurogenesis; Migration; ACTIVATED ION-CHANNEL; RADIAL GLIA; CELLS; NEURONS; COMPONENTS; MIGRATE; TBR2;
D O I
10.1007/s00429-024-02759-7
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Malformation during cortical development can disrupt the balance of excitatory and inhibitory neural circuits, contributing to various psychiatric and developmental disorders. One of the critical factors of cortical neural networks is the fine regulation of neurogenesis through mechanical cues, such as shear stress and substrate stiffness. Piezo1, a mechanically-activated channel, serves as a transducer for these mechanical cues, regulating embryogenesis. However, specific cell-type expression patterns of this channel during cortical development have not yet been characterized. In the present study, we conducted an RNAscope experiment to visualize the location of Piezo1 transcripts with embryonic neuronal/glial lineage cell markers. Our analysis covered coronal sections of the mouse forebrain on embryonic day 12.5 (E12.5), E14.5, E16.5, and E18.5. In addition, applying Yoda1, a specific Piezo1 agonist, evoked distinct calcium elevation in piriform cortices of E16.5 and E18.5 embryonic slices. Furthermore, pharmacological activation or inhibition of this channel significantly modulated the migration of neurosphere-derived cells in vitro. These findings contribute valuable insights to the field of mechanobiology and provide an understanding of the intricate processes underlying embryonic brain development.
引用
收藏
页码:759 / 773
页数:15
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