N6-methyladenosine (m6A) reader IGF2BP1 facilitates clear-cell renal cell carcinoma aerobic glycolysis

被引：14

作者：

Yuan, Bao ^{[1
]}

Zhou, Jin ^{[2
,3
]}

机构：

[1] Tianjin Beichen Tradit Chinese Med Hosp, Tianjin, Peoples R China

[2] Tianjin Med Univ, Chu Hsien I Mem Hosp, NHC Key Lab Hormones & Dev, Tianjin, Peoples R China

[3] Tianjin Med Univ, Tianjin Inst Endocrinol, Tianjin Key Lab Metab Dis, Tianjin, Peoples R China

来源：

PEERJ | 2023年 / 11卷

关键词：

ccRCC; m6A; Aerobic glycolysis; TRANSCRIPTION;

D O I：

10.7717/peerj.14591

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Emerging articles have reported that N6-methyladenosine (m6A) modification is mainly involved in clear-cell renal cell carcinoma (ccRCC) tumorigenesis. However, the regulatory mechanisms of m6A reader IGF2BP1 involved in ccRCC tumor energy metabolism are currently unknown. Results showed that the m6A reader IGF2BP1 exhibited significantly higher expression in ccRCC cells. Functionally, results by gain/loss functional assays indicated that IGF2BP1 promoted the glycolytic characteristics, including glucose uptake, lactate production and extracellular acidification rate (ECAR). Mechanistically, IGF2BP1 recognized the m6A modified sites on LDHA mRNA and enhanced its mRNA stability, thereby accelerating tumor energy metabolism. Thus, our work reveals a novel facet of the m6A that promoted mRNA stability and highlighted the functional importance of IGF2BP1 as m6A readers in post-transcriptional gene regulation.

引用

页数：12

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