共 35 条
Preparation and recognition mechanism study of an scFv targeting chloramphenicol for a hybridization chain reaction-CRISPR/Cas12a amplified fluoroimmunoassay
被引:5
作者:

Zhang, Fuyuan
论文数: 0 引用数: 0
h-index: 0
机构:
Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

Hao, Dongyue
论文数: 0 引用数: 0
h-index: 0
机构:
Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

Liu, Ruobing
论文数: 0 引用数: 0
h-index: 0
机构:
Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

Wang, Juntao
论文数: 0 引用数: 0
h-index: 0
机构:
Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

Sang, Yaxin
论文数: 0 引用数: 0
h-index: 0
机构:
Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

Wang, Shuo
论文数: 0 引用数: 0
h-index: 0
机构:
Nankai Univ, Med Coll, Tianjin 300500, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China

Wang, Xianghong
论文数: 0 引用数: 0
h-index: 0
机构:
Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China
机构:
[1] Hebei Agr Univ, Coll Food Sci & Technol, Baoding 071001, Peoples R China
[2] Nankai Univ, Med Coll, Tianjin 300500, Peoples R China
关键词:
Recombinant antibody;
Recognition mechanism;
Hybridization chain reaction;
CRISPR/Cas12a;
Immunoassay;
ANTIBODY;
D O I:
10.1016/j.aca.2024.342283
中图分类号:
O65 [分析化学];
学科分类号:
070302 ;
081704 ;
摘要:
Recombinant antibody-based immunoassays have emerged as crucial techniques for detecting antibiotic residues in food samples. Developing a stable recombinant antibody production system and enhancing detection sensitivity are crucial for their biosensing applications. Here, we bioengineered a single-chain fragment variable (scFv) antibody to target chloramphenicol (CAP) using both Bacillus subtilis and HEK 293 systems, with the HEK 293-derived scFv demonstrating superior sensitivity. Computational chemistry analyses indicated that ASP -99 and ASN-102 residues in the scFv play key roles in antibody recognition, and the hydroxyl group near the benzene ring of the target molecule is critical for in antibody binding. Furthermore, we enhanced the scFv's biosensing sensitivity using an HCR-CRISPR/Cas12a amplification strategy in a streptavidin-based immunoassay. In the dual-step amplification process, detection limits for CAP in the HCR and HCR-CRISPR/Cas12a stages were significantly reduced to 55.23 pg/mL and 3.31 pg/mL, respectively. These findings introduce an effective method for developing CAP -specific scFv antibodies and also propose a multi -amplification strategy to increase immunoassay sensitivity. Additionally, theoretical studies also offer valuable guidance in CAP hapten design and genetic engineering for antibody modification.
引用
收藏
页数:10
相关论文
共 35 条
- [1] Highly sensitive and selective electrochemical sensor for the simultaneous determination of tinidazole and chloramphenicol in food samples (egg, honey and milk)[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2023, 390Adane, Wondimeneh Dubale论文数: 0 引用数: 0 h-index: 0机构: Addis Ababa Univ, Dept Chem, POB 1176, Addis Ababa, Ethiopia Addis Ababa Univ, Dept Chem, POB 1176, Addis Ababa, EthiopiaChandravanshi, Bhagwan Singh论文数: 0 引用数: 0 h-index: 0机构: Addis Ababa Univ, Dept Chem, POB 1176, Addis Ababa, Ethiopia Addis Ababa Univ, Dept Chem, POB 1176, Addis Ababa, EthiopiaTessema, Merid论文数: 0 引用数: 0 h-index: 0机构: Addis Ababa Univ, Dept Chem, POB 1176, Addis Ababa, Ethiopia Addis Ababa Univ, Dept Chem, POB 1176, Addis Ababa, Ethiopia
- [2] A SERS microfluidic chip for ultrasensitive and simultaneous detection of SCCA and CYFRA21-1 in serum based on Au nanobowl arrays and hybridization chain reaction[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2023, 375Cao, Xiaowei论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou 225001, Peoples R China Yangzhou Univ, Med Coll, Jiangsu Key Lab Expt & Translat Noncoding RNA Res, Yangzhou 225001, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R ChinaGu, Yingyan论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China Yangzhou Univ, Northern Jiangsu Peoples Hosp, Dept Obstet & Gynecol, Yangzhou 225001, Peoples R China Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou 225001, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R ChinaLi, Zhiyue论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Northern Jiangsu Peoples Hosp, Dept Obstet & Gynecol, Yangzhou 225001, Peoples R China Dalian Med Univ, Clin Coll 1, Dalian 116011, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R ChinaGe, Shengjie论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou 225001, Peoples R China Yangzhou Univ, Med Coll, Jiangsu Key Lab Expt & Translat Noncoding RNA Res, Yangzhou 225001, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R ChinaMao, Yu论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou 225001, Peoples R China Yangzhou Univ, Med Coll, Jiangsu Key Lab Expt & Translat Noncoding RNA Res, Yangzhou 225001, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R ChinaGu, Yuexing论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou 225001, Peoples R China Yangzhou Univ, Med Coll, Jiangsu Key Lab Expt & Translat Noncoding RNA Res, Yangzhou 225001, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R ChinaLu, Dan论文数: 0 引用数: 0 h-index: 0机构: Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China Yangzhou Univ, Northern Jiangsu Peoples Hosp, Dept Obstet & Gynecol, Yangzhou 225001, Peoples R China Dalian Med Univ, Clin Coll 1, Dalian 116011, Peoples R China Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou 225001, Peoples R China Yangzhou Univ, Inst Translat Med, Med Coll, Yangzhou 225001, Peoples R China
- [3] Aptamers, antibody scFv, and antibody Fab' fragments: An overview and comparison of three of the most versatile biosensor biorecognition elements[J]. BIOSENSORS & BIOELECTRONICS, 2016, 85 : 32 - 45Crivianu-Gaita, Victor论文数: 0 引用数: 0 h-index: 0机构: Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, CanadaThompson, Michael论文数: 0 引用数: 0 h-index: 0机构: Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada Univ Toronto, Dept Chem, Toronto, ON M5S 3H6, Canada
- [4] Monoclonal antibodies (mAbs) and single chain variable fragment (scFv) antibodies targeting envelope protein VP28 of white spot syndrome virus provide protection against viral infection[J]. FISH & SHELLFISH IMMUNOLOGY, 2022, 127 : 508 - 520Cui, Chuang论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R ChinaHe, Liangyin论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R ChinaTang, Xiaoqian论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R ChinaXing, Jing论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R ChinaSheng, Xiuzhen论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R ChinaChi, Heng论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R ChinaZhan, Wenbin论文数: 0 引用数: 0 h-index: 0机构: Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China Ocean Univ China, Lab Pathol & Immunol Aquat Anim, KLMME, Qingdao 266003, Peoples R China
- [5] Development of an Immunoassay for Chloramphenicol Based on the Preparation of a Specific Single-Chain Variable Fragment Antibody[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (14) : 2971 - 2979Du, Xin-jun论文数: 0 引用数: 0 h-index: 0机构: Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R ChinaZhou, Xiao-nan论文数: 0 引用数: 0 h-index: 0机构: Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R ChinaLi, Ping论文数: 0 引用数: 0 h-index: 0机构: Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R ChinaSheng, Wei论文数: 0 引用数: 0 h-index: 0机构: Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R ChinaDucancel, Frederic论文数: 0 引用数: 0 h-index: 0机构: CEA Saclay, Pharmacol & Immune Anal Dept, F-91191 Gif Sur Yvette, France Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R ChinaWang, Shuo论文数: 0 引用数: 0 h-index: 0机构: Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China Tianjin Univ Sci & Technol, Minist Educ China, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China
- [6] A CRISPR-Cas12a powered electrochemical sensor based on gold nanoparticles and MXene composite for enhanced nucleic acid detection[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2023, 380Duan, Haowei论文数: 0 引用数: 0 h-index: 0机构: Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, Australia Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, AustraliaWang, Yizhou论文数: 0 引用数: 0 h-index: 0机构: Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, Australia Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, AustraliaTang, Shi-Yang论文数: 0 引用数: 0 h-index: 0机构: Univ Birmingham, Dept Elect Elect & Syst Engn, Birmingham B15, England Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, AustraliaXiao, Ting-Hui论文数: 0 引用数: 0 h-index: 0机构: Univ Tokyo, Dept Chem, Tokyo 1130033, Japan Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, AustraliaGoda, Keisuke论文数: 0 引用数: 0 h-index: 0机构: Univ Tokyo, Dept Chem, Tokyo 1130033, Japan Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA Wuhan Univ, Inst Technol Sci, Wuhan 430072, Hubei, Peoples R China Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, AustraliaLi, Ming论文数: 0 引用数: 0 h-index: 0机构: Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, Australia Macquarie Univ, Biomol Discovery Res Ctr, Sydney, NSW 2109, Australia Macquarie Univ, Fac Sci & Engn, Sch Engn, Sydney, NSW 2109, Australia
- [7] Immuno-HCR based on contact quenching and fluorescence resonance energy transfer for sensitive and low background detection of Escherichia coli O157:H7[J]. FOOD CHEMISTRY, 2021, 334 (334)Duan, Miaolin论文数: 0 引用数: 0 h-index: 0机构: Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaXiao, Xiaoyue论文数: 0 引用数: 0 h-index: 0机构: Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaHuang, Yanmei论文数: 0 引用数: 0 h-index: 0机构: Jiangxi YeLi Med Device Co Ltd, 2799 TianXiang Ave, Nanchang 330008, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaLi, Guoqiang论文数: 0 引用数: 0 h-index: 0机构: Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaShan, Shan论文数: 0 引用数: 0 h-index: 0机构: Jiangxi Normal Univ, 99 Ziyang Ave, Nanchang 330022, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaLv, Xi论文数: 0 引用数: 0 h-index: 0机构: Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaZhou, Houde论文数: 0 引用数: 0 h-index: 0机构: Jiangxi Prov Ctr Dis Control & Prevent, Jiangxi Prov Key Lab Diagnosing & Tracing Foodbor, 555 East Beijing Rd, Nanchang 330029, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaPeng, Silu论文数: 0 引用数: 0 h-index: 0机构: Jiangxi Prov Ctr Dis Control & Prevent, Jiangxi Prov Key Lab Diagnosing & Tracing Foodbor, 555 East Beijing Rd, Nanchang 330029, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaLiu, Chengwei论文数: 0 引用数: 0 h-index: 0机构: Jiangxi Prov Ctr Dis Control & Prevent, Jiangxi Prov Key Lab Diagnosing & Tracing Foodbor, 555 East Beijing Rd, Nanchang 330029, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaLiu, Daofeng论文数: 0 引用数: 0 h-index: 0机构: Jiangxi Prov Ctr Dis Control & Prevent, Jiangxi Prov Key Lab Diagnosing & Tracing Foodbor, 555 East Beijing Rd, Nanchang 330029, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R ChinaLai, Weihua论文数: 0 引用数: 0 h-index: 0机构: Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China Nanchang Univ, State Key Lab Food Sci & Technol, 235 East Nanjing Rd, Nanchang 330047, Jiangxi, Peoples R China
- [8] Win-Win Cooperation: Magnetic relaxation switching biosensor for chloramphenicol detection based on the pipet platform and antigen-driven enzyme-free dual signal amplification strategy[J]. SENSORS AND ACTUATORS B-CHEMICAL, 2023, 385Hong, Feng论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaXiao, Ruiheng论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaLi, Letian论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaCai, Zhaoxia论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaRen, Liangqiong论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaLi, Nan论文数: 0 引用数: 0 h-index: 0机构: Daye Publ Inspect & Test Ctr, Daye 435100, Hubei, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaZhang, Feng论文数: 0 引用数: 0 h-index: 0机构: Chinese Acad Inspect & Quarantine, Beijing 102600, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaXu, Xiuli论文数: 0 引用数: 0 h-index: 0机构: Chinese Acad Inspect & Quarantine, Beijing 102600, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R ChinaChen, Yiping论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China Guangdong Lab Lingnan Modern Agr, Guangzhou 510642, Guangdong, Peoples R China Daye Publ Inspect & Test Ctr, Daye 435100, Hubei, Peoples R China Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
- [9] An affinity improved single-chain antibody from phage display of a library derived from monoclonal antibodies detects fumonisins by immunoassay[J]. ANALYTICA CHIMICA ACTA, 2015, 867 : 74 - 82Hu, Zu-Quan论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China Guizhou Med Univ, Coll Biol & Engn, Guiyang 550004, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaLi, He-Ping论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaWu, Ping论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaLi, Ya-Bo论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaZhou, Zhu-Qing论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaZhang, Jing-Bo论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaLiu, Jin-Long论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R ChinaLiao, Yu-Cai论文数: 0 引用数: 0 h-index: 0机构: Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China Huazhong Agr Univ, Mol Biotechnol Lab Triticeae Crops, Wuhan 430070, Peoples R China
- [10] A programmable and sensitive CRISPR/Cas12a-based MicroRNA detection platform combined with hybridization chain reaction[J]. BIOSENSORS & BIOELECTRONICS, 2022, 211Jia, Hai-Yan论文数: 0 引用数: 0 h-index: 0机构: East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R ChinaZhao, Hong-Li论文数: 0 引用数: 0 h-index: 0机构: East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R ChinaWang, Ting论文数: 0 引用数: 0 h-index: 0机构: East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R ChinaChen, Pin-Ru论文数: 0 引用数: 0 h-index: 0机构: Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R ChinaYin, Bin-Cheng论文数: 0 引用数: 0 h-index: 0机构: East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832000, Xinjiang, Peoples R China East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R ChinaYe, Bang-Ce论文数: 0 引用数: 0 h-index: 0机构: East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China Zhejiang Univ Technol, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China