Sodium channel endocytosis drives axon initial segment plasticity

被引:14
作者
Freal, Amelie [1 ,2 ,4 ]
Jamann, Nora [1 ,2 ]
Ten Bos, Jolijn [2 ]
Jansen, Jacqueline [2 ]
Petersen, Naomi [1 ]
Ligthart, Thijmen [1 ]
Hoogenraad, Casper C. [2 ,3 ]
Kole, Maarten H. P. [1 ,2 ]
机构
[1] Royal Netherlands Acad Arts & Sci KNAW, Axonal Signaling Grp, Netherlands Inst Neurosci NIN, Amsterdam, Netherlands
[2] Univ Utrecht, Cell Biol Neurobiol & Biophys, Dept Biol, Fac Sci, Utrecht, Netherlands
[3] Genentech Inc, Dept Neurosci, South San Francisco, CA USA
[4] Vrije Univ VU, Amsterdam UMC, Dept Funct Genom, Amsterdam, Netherlands
关键词
ACTIVITY-DEPENDENT RELOCATION; MYOSIN-II ACTIVITY; METHYL-D-ASPARTATE; STRUCTURAL PLASTICITY; AMPA RECEPTORS; BINDING MOTIF; CA1; REGION; TRAFFICKING; MECHANISM; PROTEINS;
D O I
10.1126/sciadv.adf3885
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Activity-dependent plasticity of the axon initial segment (AIS) endows neurons with the ability to adapt action potential output to changes in network activity. Action potential initiation at the AIS highly depends on the clustering of voltage-gated sodium channels, but the molecular mechanisms regulating their plasticity remain largely unknown. Here, we developed genetic tools to label endogenous sodium channels and their scaffolding protein, to reveal their nanoscale organization and longitudinally image AIS plasticity in hippocampal neurons in slices and primary cultures. We find that N-methyl-d-aspartate receptor activation causes both long-term synaptic depression and rapid internalization of AIS sodium channels within minutes. The clathrin-mediated endocytosis of sodium channels at the distal AIS increases the threshold for action potential generation. These data reveal a fundamental mechanism for rapid activity-dependent AIS reorganization and suggests that plasticity of intrinsic excitability shares conserved features with synaptic plasticity.
引用
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页数:15
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