Development of an Online Genome Sequence Comparison Resource for Bacillus cereus sensu lato Strains Using the Efficient Composition Vector Method

被引:8
作者
Wang, Kui [1 ,2 ]
Shu, Changlong [1 ]
Bravo, Alejandra [3 ]
Soberon, Mario [3 ]
Zhang, Hongjun [4 ]
Crickmore, Neil [5 ]
Zhang, Jie [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
[2] Anhui Agr Univ, Sch Plant Protect, Hefei 230036, Peoples R China
[3] Univ Nacl Autonoma Mexico, Dept Microbiol Mol, Inst Biotecnol, Cuernavaca 62250, Mexico
[4] Minist Agr & Rural Affairs, Inst Control Agrochem, Beijing 100125, Peoples R China
[5] Univ Sussex, Sch Life Sci, Brighton BN1 9QG, England
基金
中国国家自然科学基金;
关键词
Bacillus thuringiensis; B; cereus sensu stricto; anthracis; phylogenetic analysis; composition vector tree; DNA-DNA HYBRIDIZATION; 16S RIBOSOMAL-RNA; SP; NOV; PHYLOGENY; THURINGIENSIS; ALIGNMENT; TOOL; ANNOTATION; ANTHRACIS; TAXONOMY;
D O I
10.3390/toxins15060393
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
An automated method was developed for differentiating closely related B. cereus sensu lato (s.l.) species, especially biopesticide Bacillus thuringiensis, from other human pathogens, B. anthracis and B. cereus sensu stricto (s.s.). In the current research, four typing methods were initially compared, including multi-locus sequence typing (MLST), single-copy core genes phylogenetic analysis (SCCGPA), dispensable genes content pattern analysis (DGCPA) and composition vector tree (CVTree), to analyze the genomic variability of 23 B. thuringiensis strains from aizawai, kurstaki, israelensis, thuringiensis and morrisoni serovars. The CVTree method was the best option to be used for typing B. thuringiensis strains since it proved to be the fastest method, whilst giving high-resolution data about the strains. In addition, CVTree agrees well with ANI-based method, revealing the relationship between B. thuringiensis and other B. cereus s.l. species. Based on these data, an online genome sequence comparison resource was built for Bacillus strains called the Bacillus Typing Bioinformatics Database to facilitate strain identification and characterization.
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页数:13
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