Length-Dependent Modulation of B Cell Activating Factor Transcripts in Chicken Macrophage by Viral Double-Stranded RNA

被引:2
|
作者
Abo-Samaha, Magda I. [1 ,2 ]
Sharaf, Mohammed M. [2 ]
El-Nahas, Abeer F. [2 ]
Odemuyiwa, Solomon O. [1 ,3 ]
机构
[1] Tuskegee Univ, Coll Vet Med, Dept Pathobiol, Tuskegee, AL 36088 USA
[2] Alexandria Univ, Fac Vet Med, Dept Anim Husb & Anim Wealth Dev, Alexandria, Egypt
[3] Univ Missouri, Coll Vet Med, Dept Vet Pathobiol, Columbia, MO 65211 USA
关键词
HD11; dsRNA; poly(I:C); macrophage; BAFF; chicken; RIG-I; RESPONSES; INNATE; ACID; RECOGNITION; VIRUSES; LIGAND; BAFF;
D O I
10.3390/vaccines11101561
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Viral double-stranded RNA (dsRNA) interacts with Retinoic-acid-inducible-gene-1 (RIG-1)-like receptors (RLRs) to induce type 1 interferons. Melanoma-derived-antigen-5 (MDA-5), an RLR, but not RIG-1, is found in chickens. Ducks express both RIG-1 and MDA-5, a possible cause of differences in susceptibility to influenza virus infection between chickens and ducks. Using the HD11 chicken macrophage cell line and an RT2 Profiler PCR-array system, we showed that high-molecular-weight poly(I:C), HMW-poly(I:C), upregulates CCL4, interferon-gamma, interleukin-1, and interleukin-6 mRNA transcripts. HMW-poly(I:C), an in vitro surrogate of long dsRNA species, also induces the upregulation of IL-12B and B cell activating factor (BAFF). Conversely, low-molecular-weight poly(I:C), LMW-poly(I:C) did not induce a distinct cytokine expression pattern. Nonetheless, co-transfection of LMW and HMW-poly(I:C) significantly reduced the upregulation of IL12B and BAFF by HMW-poly(I:C). These findings support previous studies that found no expression of RIG-1, a receptor for short dsRNA species, in chicken cells. Surprisingly, however, our data suggested that in the absence of RIG-1 in chicken macrophages, short dsRNA species may inhibit macrophage-mediated B cell development and survival by modulating the expression of BAFF without significantly reducing type 1 interferon response.
引用
收藏
页数:7
相关论文
共 50 条
  • [31] Double-stranded RNA is a common viral factor for NFATc1 activation leading to Th2 cytokine expression.
    Imani, F
    Kehoe, KE
    FASEB JOURNAL, 2000, 14 (06): : A925 - A925
  • [32] The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition
    Lingel, A
    Simon, B
    Izaurralde, E
    Sattler, M
    EMBO REPORTS, 2005, 6 (12) : 1149 - 1155
  • [33] Double-stranded RNA-dependent protein kinase phosphorylation of the α-subunit of eukaryotic translation initiation factor 2 mediates apoptosis
    Scheuner, Donalyn
    Patel, Rupali
    Wang, Feng
    Lee, Kuei
    Kumar, Kotlo
    Wu, Jun
    Nilsson, Anders
    Karin, Michael
    Kaufman, Randal J.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (30) : 21458 - 21468
  • [34] Length-Dependent, Single-Molecule Analysis of Short Double-Stranded DNA Fragments through Hydrogel-Filled Nanopores: A Potential Tool for Size Profiling Cell-Free DNA
    Al Sulaiman, Dana
    Gatehouse, Alfie
    Ivanov, Aleksandar P.
    Edel, Joshua B.
    Ladame, Sylvain
    ACS APPLIED MATERIALS & INTERFACES, 2021, 13 (23) : 26673 - 26681
  • [35] Involvement of double-stranded RNA-dependent protein kinase and phosphorylation of eukaryotic initiation factor-2α in neuronal degeneration
    Chang, RCC
    Suen, KC
    Ma, CH
    Elyaman, W
    Ng, HK
    Hugon, J
    JOURNAL OF NEUROCHEMISTRY, 2002, 83 (05) : 1215 - 1225
  • [36] RNase L mediates transient control of the interferon response through modulation of the double-stranded RNA-dependent protein kinase PKR
    Khabar, KSA
    Siddiqui, YM
    al-Zoghaibi, F
    al-Haj, L
    Dhalla, M
    Zhou, AM
    Dong, BH
    Whitmore, M
    Paranjape, J
    Al-Ahdal, MN
    Al-Mohanna, F
    Williams, BRG
    Silverman, RH
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (22) : 20124 - 20132
  • [37] PURIFICATION OF THE MESSENGER-RNA FOR CHICKEN VERY LOW-DENSITY LIPOPROTEINII AND MOLECULAR CLONING OF ITS FULL-LENGTH DOUBLE-STRANDED CDNA
    WIERINGA, B
    ROSKAM, W
    ARNBERG, A
    VANDERZWAAGGERRITSEN, J
    AB, G
    GRUBER, M
    NUCLEIC ACIDS RESEARCH, 1979, 7 (08) : 2147 - 2163
  • [38] A role for double-stranded RNA-dependent protein kinase, PKR in suppressing CD8 T cell responses during acute and chronic viral infection
    Nakayama, Y
    Czuprynski, C
    Williams, BRG
    Suresh, M
    FASEB JOURNAL, 2005, 19 (04): : A398 - A398
  • [39] A viral PAMP double-stranded RNA induces allergen-specific Th17 cell response in the airways which is dependent on VEGF and IL-6
    Choi, J. -P.
    Kim, Y. -S.
    Tae, Y. -M.
    Choi, E. -J.
    Hong, B. -S.
    Jeon, S. G.
    Gho, Y. S.
    Zhu, Z.
    Kim, Y. -K.
    ALLERGY, 2010, 65 (10) : 1322 - 1330
  • [40] P58IPK, a plant ortholog of double-stranded RNA-dependent protein kinase PKR inhibitor, functions in viral pathogenesis
    Bilgin, DD
    Liu, Y
    Schiff, M
    Dinesh-Kumar, SP
    DEVELOPMENTAL CELL, 2003, 4 (05) : 651 - 661