Poly(N-acryloylmorpholine) Nanogels as Promising Materials for Biomedical Applications: Low Protein Adhesion and High Colloidal Stability

被引：5

作者：

Neumann-Tran, Thi Mai Phuong ^{[1
]}

Lopez-Iglesias, Clara ^{[1
,2
,3
]}

Navarro, Lucila ^{[1
]}

Quaas, Elisa ^{[4
]}

Achazi, Katharina ^{[4
]}

Biglione, Catalina ^{[1
,5
]}

Klinger, Daniel ^{[1
]}

机构：

[1] Free Univ Berlin, Inst Pharm Pharmaceut Chem, Konigin Luise Str 2-4, D-14195 Berlin, Germany

[2] Univ Santiago De Compostela, Fac Pharm, Dept Pharmacol Pharm & Pharmaceut Technol, I D Farma Grp GI 1645,Inst Mat iMATUS, Santiago De Compostela 15782, Spain

[3] Univ Santiago de Compostela, Hlth Res Inst Santiago de Compostela IDIS, Santiago De Compostela 15782, Spain

[4] Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany

[5] IMDEA Energy Inst, Adv Porous Mat Unit, E-28935 Madrid, Spain

来源：

ACS APPLIED POLYMER MATERIALS | 2023年 / 5卷 / 10期

关键词：

nanogels; acryloylmorpholine; miniemulsion; protein repellent; drug delivery; antifoulingcoating; smart materials; ACCELERATED BLOOD CLEARANCE; NANOCOMPOSITE HYDROGELS; POLY(ETHYLENE GLYCOL); DRUG-RELEASE; PH; POLYMERIZATION; PARTICLES; POLYMERS; ACRYLOYLMORPHOLINE; NANOMEDICINE;

D O I：

10.1021/acsapm.3c00890

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Poly(N-acryloylmorpholine) (P(NAM))-based materials have been developed to prevent protein adhesion to surfaces due to their biocompatibility and protein-repellent properties. However, transferring the benefits of P(NAM) to nanoscale materials such as nanogels has not yet been studied. This can be attributed to the challenging colloidal synthesis of such particles with highly hydrophilic networks. To address this challenge, we have developed an inverse miniemulsion approach for free radical polymerization of NAM in dispersed nanodroplets. This strategy allows preparation of well-defined P(NAM) nanogels with a controllable size (250-350 nm). To impart additional functionality, our approach can easily be adapted to include ionic co-monomers and degradable cross-linkers. The resulting pH-responsive swelling and redox-triggered degradation profiles were demonstrated by dynamic light scattering measurements. To test the influence of such additional functionality on the protein-repellent properties, protein adsorption on the nanogels was assessed. For surface-immobilized nanogels, reduced unspecific binding of albumin was demonstrated for all nanogels via fluorescence microscopy. For nanogels in suspension, nanoparticle tracking analysis showed no increase in nanogel size upon incubation in serum and plasma, thus suggesting limited protein adsorption and colloidal high stability for over 2 months. Finally, cytotoxicity essays demonstrated the potential of these materials for bio-applications. Overall, these results suggest that biocompatibility and protein-repellent properties of P(NAM) can be transferred to nanogels and are maintained upon integration of additional chemical functionality. Thus, our synthetic strategy builds the foundation for utilizing such versatile colloidal materials in biomedical applications, e.g., as versatile drug delivery systems.

引用

页码：7718 / 7732

页数：15