Circulating miRNA Expression Is Inversely Correlated with Tumor Tissue or Sentinel Lymph Nodes in Estrogen Receptor-Positive Early Breast Cancer Patients

被引:2
作者
Escuin, Daniel [1 ]
Lopez-Vilaro, Laura [1 ,2 ]
Bell, Olga [1 ]
Mora, Josefina [3 ]
Garcia-Valdecasas, Barbara [4 ]
Moral, Antonio [4 ,5 ]
Clos, Montserrat [4 ]
Boronat, Laia [6 ]
Arqueros, Cristina [6 ]
Barnadas, Agusti [1 ,5 ,6 ,7 ]
机构
[1] Inst Invest Biomed St Pau IIB St Pau, Barcelona, Spain
[2] Hosp Santa Creu & Sant Pau, Dept Pathol, Barcelona, Spain
[3] Hosp Univ Santa Creu & St Pau, Dept Biochem, Barcelona, Spain
[4] Hosp Santa Creu & Sant Pau, Dept Surg, Barcelona, Spain
[5] Univ Autonoma Barcelona UAB, Fac Med, Bellaterra 08193, Spain
[6] Hosp Santa Creu & Sant Pau, Dept Med Oncol, Barcelona 08041, Spain
[7] Ctr Invest Biomed Red Canc CIBERONC, Madrid 28029, Spain
关键词
miRNAs; early breast cancer; sentinel lymph node; metastasis; biomarkers; DIFFERENTIAL EXPRESSION; MICRORNAS; PACKAGE; MARKERS; PLASMA;
D O I
10.3390/ijms241713293
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The deregulation of microRNAs (miRNAs) is associated with the various steps of the metastatic process. In addition, circulating miRNAs are remarkably stable in peripheral blood, making them ideal noninvasive biomarkers for disease diagnosis. Here, we performed a proof-of-principle study to determine whether tumor-tissue-derived miRNAs are traceable to plasma in ER-positive early breast cancer patients. We performed RNA-sequencing on 30 patients for whom plasma, sentinel lymph nodes (SLNs) and tumor tissue were available. We carried out differential expression, gene ontology and enrichment analyses. Our results show that circulating miRNAs are inversely expressed compared with tumor tissue or SLNs obtained from the same patients. Our differential expression analysis shows the overall downregulation of circulating miRNAs. However, the expression of miR-643a-3p and miR-223 was up-regulated in patients with positive SLNs. Furthermore, gene ontology analysis showed the significant enrichment of biological processes associated with the regulation of epithelial cell proliferation and transcriptional regulation commonly involved in the promotion of metastases. Our results suggest the potential role of several circulating miRNAs as surrogate markers of lymph node metastases in early breast cancer patients. Further preclinical and clinical studies are required to understand the biological significance of the most significant miRNAs and to validate our results in a larger cohort of patients.
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页数:14
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