Volumetric Temperature Mapping Using Light-Sheet Microscopy and Upconversion Fluorescence from Micro- and Nano-Rare Earth Composites

被引：1

作者：

Barron-Ortiz, Dannareli ^{[1
]}

Cadena-Nava, Ruben D. ^{[2
]}

Perez-Parets, Enric ^{[3
]}

Licea-Rodriguez, Jacob ^{[4
]}

Gualda, Emilio J. ^{[3
,5
]}

Hernandez-Cordero, Juan ^{[6
]}

Loza-Alvarez, Pablo ^{[3
]}

Rocha-Mendoza, Israel ^{[1
]}

机构：

[1] Ctr Invest Cient & Educ Super Ensenada CICESE, Ensenada Tijuana 3918, Ensenada 22860, Mexico

[2] Univ Nacl Autonoma Mexico, Ctr Nanociencias & Nanotecnol CNyN, Km 107 Carretera Tijuana Ensenada, Ensenada 22860, Mexico

[3] Barcelona Inst Sci & Technol, ICFO Inst Ciencies Foton, Av Carl Friedrich Gauss 3, Castelldefels 08860, Spain

[4] Univ Autonoma Estado Morelos, Ctr Invest Ingn & Ciencias Aplicadas CIICAp, Cuernavaca 62209, Mexico

[5] Univ Politecn Cataluna, Dept Agrifood Engn & Biotechnol DEAB, Dept Fis & Engn Nucl, Esteve Terradas 8, Castelldefels 08860, Spain

[6] Univ Nacl Autonoma Mexico, Inst Invest Mat, AP 70-360, Mexico City 04510, Mexico

来源：

MICROMACHINES | 2023年 / 14卷 / 11期

关键词：

light-sheet microscopy; upconversion fluorescence; 3D imaging; temperature mapping; LUMINESCENCE; ER3+; NANOPARTICLES; EMISSION; SENSORS;

D O I：

10.3390/mi14112097

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a combination of light-sheet excitation and two-dimensional fluorescence intensity ratio (FIR) measurements as a simple and promising technique for three-dimensional temperature mapping. The feasibility of this approach is demonstrated with samples fabricated with sodium yttrium fluoride nanoparticles co-doped with rare-earth ytterbium and erbium ions (NaYF4:Yb3+/Er3+) incorporated into polydimethylsiloxane (PDMS) as a host material. In addition, we also evaluate the technique using lipid-coated NaYF4:Yb3+/Er3+ nanoparticles immersed in agar. The composite materials show upconverted (UC) fluorescence bands when excited by a 980 nm near-infrared laser light-sheet. Using a single CMOS camera and a pair of interferometric optical filters to specifically image the two thermally-coupled bands (at 525 and 550 nm), the two-dimensional FIR and, hence, the temperature map can be readily obtained. The proposed method can take optically sectioned (confocal-like) images with good optical resolution over relatively large samples (up to the millimetric scale) for further 3D temperature reconstruction.

引用

页数：14