GCaMP, a Family of Single-Fluorophore Genetically Encoded Calcium Indicators

被引：4

作者：

Erofeev, A. I. ^{[1
]}

Vinokurov, E. K. ^{[1
]}

Vlasova, O. L. ^{[1
]}

Bezprozvanny, I. B. ^{[1
,2
]}

机构：

[1] Peter Great St Petersburg Polytech Univ, Inst Biomed Syst & Biotechnol, Lab Mol Neurodegenerat, St Petersburg, Russia

[2] Univ Texas Southwestern Med Ctr Dallas, Dept Physiol, Dallas, TX USA

来源：

JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY | 2023年 / 59卷 / 04期

基金：

俄罗斯科学基金会;

关键词：

calcium indicators; neuronal activity; dynamics; fluorescence; GREEN FLUORESCENT PROTEINS; IMAGING LIGHT RESPONSES; IN-VIVO; NEURAL ACTIVITY; NEURONAL-ACTIVITY; MOLECULAR-MECHANISMS; CELLULAR RESOLUTION; CA2+ INDICATORS; TRANSGENIC MICE; DYNAMICS;

D O I：

10.1134/S0022093023040142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Single-fluorophore genetically encoded calcium indicators(GECIs), such as GCaMP, are widely used tools to investigate neuronalactivity. Their primary advantage lies in their capability to providereal-time and highly sensitive responses to fluctuations in intracellularCa(2+) concentrations. This property is of particular importance whenstudying neuronal processes and ensembles wherein calcium signalsplay a crucial role in information transmission. This comprehensivereview focuses on the GCaMP family, encompassing an analysis oftheir various types, distinctive features, and potential applicationsfor visualizing neuronal activity. Special attention is given tothe ongoing advancements in GCaMP technology, specifically, theendeavors to expand their spectral properties and enhance theircapability to detect high-frequency spike activity.

引用

页码：1195 / 1214

页数：20

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