Effects of Lactobacillus casei NCU011054 on immune response and gut microbiota of cyclophosphamide induced immunosuppression mice

被引：25
作者：
Min, Fangfang [1 ,2 ]
Hu, Jielun [1 ]
Huang, Tao [3 ]
Huang, Yousheng [2 ]
Nie, Shaoping [1 ]
Xiong, Tao [1 ]
Xie, Mingyong [1 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Key Lab Bioact Polysaccharides Jiangxi Prov, China Canada Joint Lab Food Sci & Technol Nanchang, 235 Nanjing East Rd, Nanchang 330047, Peoples R China
[2] Inst Dev & Res, Jiangxi Gen Inst Testing & Certificat, 171 Beijing East Rd, Nanchang 330029, Peoples R China
[3] Nanchang Univ, Int Inst Food Innovat, Xiaolan Econ & Technol Dev Zone, Luozhu Rd, Nanchang 330052, Peoples R China
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2023年 / 174卷
基金：
中国国家自然科学基金;
关键词：
Lactobacillus casei NCU011054; Immunoregulation; Gut microbiota; Metabolites; Th1; Th2; balance; PLANTARUM NCU116; BARRIER; DIFFERENTIATION; MODULATION; ACTIVATION; CYTOKINES; SYSTEM; INJURY; CELLS;
D O I：
10.1016/j.fct.2023.113662
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Lactobacillus (L.) casei NCU011054 isolated from infant feces has been proven to be a potential probiotic in vitro. The present study aimed to investigate the effects of L. casei NCU011054 on the immune response and gut microbiota in cyclophosphamide (CP)-induced immunosuppression mice. Results indicated that L. casei NCU011054 could increase the levels of mucin (Muc2) and tight junction proteins (ZO-1, occludin and claudin-1). Moreover, L. casei NCU011054 was found to upregulate TLRs/NF-kappa B pathway (TLR-2, TLR-4, TLR-6, p65 and NF-kappa B) and two transcription factors (T-bet and GATA-3) mRNA levels, and enhance the number of CD4+T cells. Th1-related cytokines (IL-12p70, IFN-gamma and TNF-alpha) and Th2-related cytokines (IL-2, IL-4, IL-6 and IL-10) significantly increased after L. casei NCU011054 treatment. More importantly, L. casei NCU011054 increased the ratio of T-bet to GATA-3 and IFN-gamma to IL-4. Apart from these, L. casei NCU011054 remodeled gut microbiota and modulated gut metabolites in CP-induced immunosuppressed mice. The correlation analysis showed that Lactobacillus upregulated by L. casei NCU011054 was positively correlated with TLRs/NF-kappa B pathway, and the ratio of T-bet to GATA-3 and IFN-gamma to IL-4. All findings revealed that L. casei NCU011054 could improve in-testinal immune dysfunction and modulate Th1/Th2 balance via TLRs/NF-kappa B pathway in CP-induced immu-nosuppressed mice.
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页数：13
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