Apoptosis-inducing, anti-angiogenic and anti-migratory effects of a dinuclear Pd(II) complex on breast cancer: A promising novel compound

被引:1
作者
Genel, Merve Erkisa [1 ,2 ]
Adacan, Kaan [1 ,3 ]
Selvi, Selin [1 ]
Kutucu, Deniz Erol
Uvez, Ayca [5 ]
Armutak, Elif Ilkay [5 ]
Sengul, Abdurrahman [6 ]
Ulukaya, Engin [1 ,7 ]
Gurevin, Ebru Gurel [4 ]
机构
[1] Istinye Univ, Mol Canc Res Ctr ISUMKAM, Istanbul, Turkiye
[2] Istanbul Hlth & Technol Univ, Fac Med, Dept Med Biol, Istanbul, Turkiye
[3] Istinye Univ, Dept Mol Biol & Genet, Fac Sci & Art, Istanbul, Turkiye
[4] Istanbul Univ, Fac Sci, Dept Biol, Istanbul, Turkiye
[5] Istanbul Univ Cerrahpasa, Fac Vet Med, Dept Hist & Embryol, Istanbul, Turkiye
[6] Bulent Ecevit Univ, Fac Sci & Art, Dept Chem, Zonguldak, Turkiye
[7] Istinye Univ, Fac Med, Dept Clin Biochem, Istanbul, Turkiye
关键词
Breast cancer; Apoptosis; Palladium; CAM assay; Invasion; PLATINUM(II) SACCHARINATE COMPLEXES; OXIDATIVE DNA-DAMAGE; PALLADIUM(II) COMPLEXES; IN-VITRO; BIOLOGICAL EVALUATION; CYTOTOXIC ACTIVITY; MEMBRANE; AUTOPHAGY; PT(II); ASSAY;
D O I
10.1016/j.mvr.2023.104619
中图分类号
R6 [外科学];
学科分类号
1002 ; 100210 ;
摘要
Because of the high mortality and morbidity rate of breast cancer, successful management of the disease requires synthesis of novel compounds. To this end, ongoing attempts to create new candidates include synthesis of multinuclear metal complexes. The high DNA binding affinity and cytotoxic activity of these complexes makes them promising as breast cancer treatments. This study investigated anti-growth/cytotoxic effect of the dinuclear Pd(II) complex on breast cancer cell lines (MCF-7, MDA-MB-231) using various methods of staining, flow cytometry, and immunoblotting. The study conducted colony formation, invasion, and migration assays were to assess the effect of the complex on metastasis. Increased caspase-3/7 levels and positive annexin V staining were observed in both cell lines, proving apoptosis. Altered TNFR1 and TRADD expression with caspase-8 cleavage followed by BCL-2 inactivation with loss of mitochondrial membrane potential confirmed the presence of apoptosis in MCF-7 and MDA-MB-231, regardless of p53 expression status. The results implied anti-migration properties. Finally, the study used the CAM assay to assess antiangiogenic properties and showed that the complex inhibited angiogenesis. The study concluded the dinuclear Pd(II) complex warrants further in vivo experiments to show its potential in the treatment of breast cancer.
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页数:12
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