The expanding vistas of spatial transcriptomics

被引：208

作者：

Tian, Luyi ^{[1
]}

Chen, Fei ^{[1
,2
]}

Macosko, Evan Z. ^{[1
,3
]}

机构：

[1] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[2] Harvard Stem Cell & Regenerat Biol, Cambridge, MA 02138 USA

[3] Massachusetts Gen Hosp, Dept Psychiat, Boston, MA 02114 USA

来源：

NATURE BIOTECHNOLOGY | 2023年 / 41卷 / 06期

关键词：

GENOME-WIDE EXPRESSION; IN-SITU; GENE-EXPRESSION; CELL ATLAS; RNA; SEQ; VISUALIZATION; LOCALIZATION; PLATFORM; TISSUE;

D O I：

10.1038/s41587-022-01448-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The formation and maintenance of tissue integrity requires complex, coordinated activities by thousands of genes and their encoded products. Until recently, transcript levels could only be quantified for a few genes in tissues, but advances in DNA sequencing, oligonucleotide synthesis and fluorescence microscopy have enabled the invention of a suite of spatial transcriptomics technologies capable of measuring the expression of many, or all, genes in situ. These technologies have evolved rapidly in sensitivity, multiplexing and throughput. As such, they have enabled the determination of the cell-type architecture of tissues, the querying of cell-cell interactions and the monitoring of molecular interactions between tissue components. The rapidly evolving spatial genomics landscape will enable generalized high-throughput genomic measurements and perturbations to be performed in the context of tissues. These advances will empower hypothesis generation and biological discovery and bridge the worlds of tissue biology and genomics. Spatial transcriptomics workflows, metrics and limitations are reviewed and discussed.

引用

页码：773 / 782

页数：10

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