Celastrol suppresses human pancreatic cancer via m6A-YTHDF3-mediated downregulation of Claspin and Bcl-2

被引：1

作者：

Zhou, Yang ^{[1
]}

Zhuang, Haoran ^{[1
]}

Liu, Yuxiang ^{[1
]}

Yin, Jing ^{[1
]}

Wei, Xiaoying ^{[1
]}

Qiu, Yue ^{[1
]}

Tian, Zhen ^{[1
]}

Miao, Tingyu ^{[1
]}

Chen, Jing ^{[1
]}

Li, Peifen ^{[1
]}

Xu, Xiao ^{[2
]}

Wu, Wenjuan ^{[3
]}

Li, Huanan ^{[1
]}

Shen, Weigan ^{[1
,4
]}

机构：

[1] Yangzhou Univ, Sch Med, Dept Cell Biol, Yangzhou, Jiangsu, Peoples R China

[2] Taizhou Hosp Tradit Chinese Med, Dept Oncol, Taizhou, Jiangsu, Peoples R China

[3] Yangzhou Univ, Clin Med Coll, Dept Oncol, Yangzhou, Jiangsu, Peoples R China

[4] Yangzhou Univ, Jiangsu Key Lab Integrated Tradit Chinese & Wester, Yangzhou, Jiangsu, Peoples R China

来源：

DISCOVER ONCOLOGY | 2023年 / 14卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Celastrol; RNA m(6)A modification; Pancreatic cancer; Claspin; APOPTOSIS; PROLIFERATION;

D O I：

10.1007/s12672-023-00838-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background Celastrol has been revealed to exhibit anticancer pharmacological activity, however, the molecular mechanisms of celastrol involved in pancreatic cancer remain to be further elucidated. The present study was to illustrate whether celastrol suppresses pancreatic cancer through modulating RNA m(6)A modification.Methods Effect of celastrol treatment on the malignant phenotypes of pancreatic cancer cells was evaluated by CCK-8 assay, EdU assay, colony formation assay, flow cytometry analysis and subcutaneous xenograft experiments. RNA sequencing (RNA-seq) analysis was carried out to analyze the genes differentially expressed in celastrol-treated pancreatic cancer cells. RT-qPCR, Western blotting and immunohistochemistry were employed to evaluate the expression of the indicated genes. RNA dot blot and quantification of total RNA m(6)A modification assays, MeRIP-qPCR assay, RIP-qPCR assay, RNA stability and protein stability assays were applied to evaluate the regulatory mechanism of celastrol treatment in pancreatic cancer cells.Results We demonstrated that celastrol suppressed cell proliferation and induced cell cycle arrest and apoptosis of pancreatic cancer cells in vitro, and decreased tumor growth in vivo. Specifically, Bcl-2, Claspin, METTL3 and YTHDF3 were identified as the potential targets of celastrol treatment in pancreatic cancer cells. Moreover, our results indicated that celastrol treatment downregulated METTL3 and decreased m(6)A levels of Claspin and Bcl-2 mRNA, leading to the degradation of Claspin and Bcl-2 mRNA in pancreatic cancer cells. Furthermore, we revealed that celastrol treatment downregulated Claspin and Bcl-2, at least in part, in an m(6)A -YTHDF3-mediated manner in pancreatic cancer cells.Conclusion Our study highlighted a novel mechanism underlying celastrol-induced cellular proliferation inhibition and apoptosis in pancreatic cancer cells via m(6)A -YTHDF3-mediated downregulation of Claspin and Bcl-2.

引用

页数：16

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