Discriminating Glioblastoma from Peritumoral Tissue by a Nanohole Array-Based Optical and Label-Free Biosensor

被引：4

作者：

Garcia-Milan, Victor ^{[1
,2
]}

Franco, Alfredo ^{[3
,4
]}

Zvezdanova, Margarita Estreya ^{[4
]}

Marcos, Sara ^{[5
]}

Martin-Laez, Ruben ^{[1
,2
]}

Moreno, Fernando ^{[3
,4
]}

Velasquez, Carlos ^{[1
,2
,4
,6
]}

Fernandez-Luna, Jose L. ^{[4
,7
]}

机构：

[1] Hosp Univ Marques de Valdecilla, Dept Neurol Surg, Santander 39008, Spain

[2] Hosp Univ Marques de Valdecilla, Spine Unit, Santander 39008, Spain

[3] Univ Cantabria, Fac Sci, Dept Appl Phys, Santander 39005, Spain

[4] Inst Invest Marques de Valdecilla IDIVAL, Santander 39012, Spain

[5] Hosp Univ Marques de Valdecilla, Serv Anat Patol, Santander 39008, Spain

[6] Univ Cantabria, Dept Anat & Cell Biol, Santander 39005, Spain

[7] Hosp Univ Marques de Valdecilla, Genet Unit, Santander 39008, Spain

来源：

BIOSENSORS-BASEL | 2023年 / 13卷 / 06期

关键词：

glioblastoma; biosensor; extraordinary optical transmission; refractive index; CENTRAL-NERVOUS-SYSTEM; PROSPECTIVE PHASE-II; INTRAOPERATIVE MRI; 5-AMINOLEVULINIC ACID; GUIDED RESECTION; SURGERY; CLASSIFICATION; GLIOMAS; TUMORS;

D O I：

10.3390/bios13060591

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In glioblastoma (GBM) patients, maximal safe resection remains a challenge today due to its invasiveness and diffuse parenchymal infiltration. In this context, plasmonic biosensors could potentially help to discriminate tumor tissue from peritumoral parenchyma based on differences in their optical properties. A nanostructured gold biosensor was used ex vivo to identify tumor tissue in a prospective series of 35 GBM patients who underwent surgical treatment. For each patient, two paired samples, tumor and peritumoral tissue, were extracted. Then, the imprint left by each sample on the surface of the biosensor was individually analyzed, obtaining the difference between their refractive indices. The tumor and non-tumor origins of each tissue were assessed by histopathological analysis. The refractive index (RI) values obtained by analyzing the imprint of the tissue were significantly lower (p = 0.0047) in the peritumoral samples (1.341, Interquartile Range (IQR) 1.339-1.349) compared with the tumor samples (1.350, IQR 1.344-1.363). The ROC (receiver operating characteristic) curve showed the capacity of the biosensor to discriminate between both tissues (area under the curve, 0.8779, p < 0.0001). The Youden index provided an optimal RI cut-off point of 0.003. The sensitivity and specificity of the biosensor were 81% and 80%, respectively. Overall, the plasmonic-based nanostructured biosensor is a label-free system with the potential to be used for real-time intraoperative discrimination between tumor and peritumoral tissue in patients with GBM.

引用

页数：13