Eco-friendly Synthesis of Zinc Oxide Nanoparticles by Garcinia cambogia and Evaluation of Their Obesity and Antimicrobial Activities

In the present synthesis, study of nanoparticles of zinc oxide using ethanolic extract of Garcinia cambogia leaves extract. Using of zinc oxide nanoparticles as a precursor in leaf extracts of this plant for NPs synthesis. The optical properties and structural of NPs were investigated by ultraviolet-visible(UV-Vis) spectrophotometer ,scanning electron microscope (SEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS).Liquid Chromatography-Electrospray Ionization-Mass (LC-ESI-Mass) in negative ion mode of ethanolic extract demonstrates the presence of six bioactive compounds identified as guanosine, prephenic acid, tetrahydroxycholanic acid, limocitrin, 6,7-dihydroxycoumarin-6-glucoside and hydroxy citric acid which they responsible for providing electrons for reducing zinc nitrate hexahydrate to ZnO-NPs.The antibacterial activity of both ethanolic extract and eco-friendly synthesis Of ZnO-NPs. by G.cambogia were tested against two pathogenic bacterial strains ,Staphylococcus aureus ATCC6538 and Escherichia coli ATCC10536 by using disk diffusion method.The ZnO-NPs showed antibacterial activity against S. aureus (40 mm) more than ethanolic extract (35 mm). It revealed significant antibacterial activity against E. coli (50 mm) compared to ethanolic extract (45 mm). Also, ZnO-NPs displayed more antibacterial activity than Ampicillin against S. aureus (266.6%) and E. coli (500%) and displayed more antifungal activity than Fluconazole against C. albicans (238.6%). However, ZnO-NPs showed higher antifungal activity against C.albicans (50.11 mm) than the ethanolic extract (45.37 mm).Based on these findings, it is concluded that the synthesized ZnO-NPs is a more promising candidate than the alcoholic extract of G. Cambogia leaves against pathogenic bacterial, fungal strains.The administration of G. cambogia and synthesized ZnO-Nps as a weight-reduction treatment is intended to give the important knowledge for both scholarly and broad public use regarding the effects of the plant.