RNA-seq analysis of human trabecular endothelial cells after treatment with timolol maleate

被引:1
作者
Palchunova, Kseniya [1 ]
Kaji, Yuichi [1 ,2 ]
Fujita, Akari [3 ]
Oshika, Tetsuro [1 ]
机构
[1] Univ Tsukuba Majors Med Sci, Dept Ophthalmol, 1-1-1 Tennoudai, Tsukuba, Ibaraki 3058575, Japan
[2] Matsumoto Eye Clin, 2-25-2F Toride I Ctr,Chuo Machi, Toride, Ibaraki 3020014, Japan
[3] Kagurazaka Eye Clin, 115 Yarai Cho Shinjyuku, Tokyo 1620805, Japan
关键词
Timolol maleate; Trabecular endothelial cells; RNA-seq; Matrix metalloproteinase; VGF nerve growth factor inducible; OPEN-ANGLE GLAUCOMA; GENE-EXPRESSION PROFILE; EXTRACELLULAR-MATRIX; AQUEOUS-HUMOR; MESHWORK; EPINEPHRINE; MECHANISM; PRESSURE;
D O I
10.1007/s10384-023-00998-5
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PurposeTimolol maleate (timolol), a beta-receptor blocker, reduces intraocular pressure by decreasing aqueous humor production. Timolol reportedly also protects ganglion cells, decreases aqueous humor outflow facility, and destroys the extracellular matrix in the trabecular meshwork. In this study, we investigated the effects of timolol on cultured human trabecular endothelial cells purchased from ScienCell using next-generation sequencing.Study designExperimental investigation.MethodsTotal ribonucleic acid (RNA) was extracted after 24 h. More than 100 million RNAs in control and timolol-treated group were sequenced using a next-generation sequencer. The expression of 55,778 RNAs was analyzed.ResultsA total of 2,105 genes were significantly upregulated and 2,125 genes were downregulated, after the addition of timolol. VGF nerve growth factor inducible (VGF) (388-fold) had the maximum increase in expression, followed by amphiregulin (333-fold), a member of the epidermal growth factor family. Moreover, the expression of extracellular matrix-degrading enzymes, matrix metalloproteinases (MMPs) 1, 2, 3, 10, 12, and 14, increased.ConclusionTimolol exerts various effects on human trabecular endothelial cells. The increase in MMP expression may contribute to the decrease in the aqueous humor outflow facility.
引用
收藏
页码:440 / 447
页数:8
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