In Vitro and In Vivo Anti-Inflammatory Effects of Cannabidiol Isolated from Novel Hemp (Cannabis sativa L.) Cultivar Pink Pepper

被引：3
作者：
Kim, Jong-Hui [1 ]
Hong, Min [1 ]
Han, Joon-Hee [1 ]
Ryu, Byeong Ryeol [2 ]
Lim, Young Seok [2 ,3 ]
Lim, Jung Dae [2 ,4 ]
Kim, Chang Hyeug [1 ]
Lee, Soo-Ung [1 ]
Kwon, Tae-Hyung [1 ]
机构：
[1] Chuncheon Bioind Fdn, Inst Biol Resources, Chunchon 24232, South Korea
[2] Kangwon Natl Univ, Grad Sch, Dept Biohlth Convergence, Chunchon 24341, South Korea
[3] Kangwon Natl Univ, Dept Biohlth Technol, Chunchon 24341, South Korea
[4] Kangwon Natl Univ, Dept Herbal Med Resource, Samcheok 25949, South Korea
来源：
MOLECULES | 2023年 / 28卷 / 18期
关键词：
anti-inflammatory activity; cannabidiol; Cannabis sativa; edema; RAW; 264.7; cells; PARALLEL-GROUP; DOUBLE-BLIND; CANCER; MAPK; ACTIVATION; EXPRESSION; RECEPTORS; MUSCLE;
D O I：
10.3390/molecules28186439
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Cannabis sativa L. contains more than 80 cannabinoids, among which cannabidiol (CBD) is the main neuroactive component. We aimed to investigate the anti-inflammatory efficacy of CBD in vitro and in vivo isolated from "Pink pepper", a novel hemp cultivar, by repeating the method of selecting and cultivating individuals with the highest CBD content. We investigated the effects of CBD on inflammatory markers elevated by lipopolysaccharide (LPS) treatment in RAW 264.7 mouse macrophage cells through Western blot and RT-PCR. In addition, we confirmed these effects through the ELISA of inflamed paw tissue of a lambda-carrageenan-induced mouse edema model that received an oral administration of CBD. CBD inhibited the LPS-induced phosphorylation of NF-kappa B and MAPK in RAW 264.7 and exhibited anti-inflammatory effects by participating in these pathways. In our in vivo study, we confirmed that CBD also inhibited the inflammatory mediators of proteins extracted from edematous mouse paw tissue. These results show that CBD isolated from "Pink pepper" exhibits potent anti-inflammatory effects. These anti-inflammatory effects of CBD have pharmacological and physiological significance, highlighting the industrial value of this novel cultivar.
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页数：15
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