Facilitation of Behavioral and Cortical Emergence from Isoflurane Anesthesia by GABAergic Neurons in Basal Forebrain

被引:14
作者
Cai, Ping [1 ,2 ]
Su, Wei-Kun [1 ]
Zhang, Jin-Sheng [1 ]
Liu, Pei-Chang [3 ]
Liu, Feng [4 ]
Liu, Ren-Fu [5 ]
Li, Zhang-Shu [5 ]
Zhu, Zhong-Hua [5 ]
Xiao, Wen-Hao [5 ]
Hu, Yong-Huai [5 ]
Cai, Hong-Da [6 ]
Wu, Xiao-Dan [4 ]
Zhang, Liang-Cheng [3 ]
Yu, Changxi [1 ,7 ]
Chen, Li [1 ,7 ]
机构
[1] Fujian Med Univ, Sch Pharm, Dept Pharmacol, Fuzhou 350108, Fujian, Peoples R China
[2] Fujian Med Univ, Sch Publ Hlth, Fujian Prov Key Lab Environm & Hlth, Fuzhou 350108, Fujian, Peoples R China
[3] Fujian Med Univ, Dept Anesthesiol, Union Hosp, Fuzhou 350001, Fujian, Peoples R China
[4] Fujian Med Univ, Fujian Prov Hosp, Dept Anesthesiol, Shengli Clin Med Coll, Fuzhou 350001, Fujian, Peoples R China
[5] Fujian Med Univ, Sch Basic Med Sci, Fuzhou 350108, Fujian, Peoples R China
[6] Fujian Med Univ, Affiliated Hosp 1, Dept Anesthesiol, Fuzhou 350001, Fujian, Peoples R China
[7] Fujian Key Lab Drug Target Discovery & Struct & Fu, Fuzhou 350108, Fujian, Peoples R China
基金
中国国家自然科学基金;
关键词
basal forebrain; fiber photometry; GABAergic neurons; general anesthesia; isoflurane anesthesia; optogenetics; PARVALBUMIN NEURONS; CHOLINERGIC NEURONS; SLEEP; CIRCUIT; ACTIVATION; MECHANISMS;
D O I
10.1523/JNEUROSCI.0628-22.2023
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
General anesthesia shares many similarities with natural sleep in behavior and electroencephalogram (EEG) patterns. The latest evidence suggests that general anesthesia and sleep-wake behavior may share overlapping neural substrates. The GABAergic neurons in the basal forebrain (BF) have recently been demonstrated to play a key role in controlling wakefulness. It was hypothesized that BF GABAergic neurons may participate in the regulation of general anesthesia. Here, using in vivo fiber photometry, we found that the activity of BF GABAergic neurons was generally inhibited during isoflurane anesthesia, having obviously decreased during the induction of anesthesia and being gradually restored during the emergence from anesthesia, in Vgat-Cre mice of both sexes. Activation of BF GABAergic neurons with chemogenetic and optogenetic approaches decreased sensitivity to isoflurane, delayed induction, and accelerated emergence from isoflurane anesthesia. Optogenetic activation of BF GABAergic neurons decreased EEG d power and the burst suppression ratio (BSR) during 0.8% and 1.4% isoflurane anesthesia, respectively. Similar to the effects of activating BF GABAergic cell bodies, photostimulation of BF GABAergic terminals in the thalamic reticular nucleus (TRN) also strongly promoted cortical activation and behavioral emergence from isoflurane anesthesia. Collectively, these results showed that the GABAergic BF is a key neural substrate for general anesthesia regulation that facilitates behavioral and cortical emergence from general anesthesia via the GABAergic BF-TRN pathway. Our findings may provide a new target for attenuating the depth of anesthesia and accelerating emergence from general anesthesia.
引用
收藏
页码:2907 / 2920
页数:14
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