Functional Analysis of the Anthocyanin-Associated Glutathione S-Transferase Gene StGST1 in Potato

被引：2

作者：

Zhang, Zhonghua ^{[1
]}

Zhang, Huiling ^{[1
]}

Liu, Tengfei ^{[2
,3
]}

Zhao, Yanan ^{[1
]}

Zhao, XiJuan ^{[4
]}

Liu, Ju ^{[1
]}

Zhang, Juping ^{[1
]}

机构：

[1] Henan Univ Sci & Technol, Coll Hort & Plant Protect, Luoyang 471000, Peoples R China

[2] Huazhong Agr Univ, Key Lab Hort Plant Biol, Minist Educ, Wuhan 430070, Peoples R China

[3] Huazhong Agr Univ, Key Lab Potato Biol & Biotechnol, Minist Agr & Rural Affairs, Wuhan 430070, Peoples R China

[4] Hunan Agr Univ, Coll Hort, Changsha 410128, Peoples R China

来源：

POTATO RESEARCH | 2023年 / 66卷 / 01期

关键词：

Anthocyanin; Potato; StGST1; Transcription activation; BIOSYNTHESIS; TRANSPORT; PROTEIN;

D O I：

10.1007/s11540-022-09582-9

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Anthocyanins are biosynthesized on the cytoplasmic surface of the endoplasmic reticulum and then transported to the vacuole for storage. Glutathione S-transferases (GSTs) are involved in the transport of anthocyanins. However, the regulatory mechanism of GSTs in potato is unclear. In this study, the glutathione S-transferase gene StGST1 was isolated from potato and characterized. Expression analysis revealed that StGST1 transcript levels were higher in pigmented tubers than in white tubers. Overexpression of StGST1 promoted anthocyanin accumulation in the tuber. Transient luciferase analysis revealed that the promoter of StGST1 was activated by the transcription factors StAN2 and StAN11. These results indicated that StGST1 promotes anthocyanin accumulation in potato tubers. These findings have important implications for improvement of anthocyanin accumulation in pigmented potato tubers.

引用

页码：215 / 230

页数：16

共 30 条

[1] Antioxidants in potato [J].

Brown, CR .

AMERICAN JOURNAL OF POTATO RESEARCH, 2005, 82 (02) :163-172

[2] Variation of anthocyanin and carotenoid contents and associated antioxidant values in potato breeding lines [J].

Brown, CR ;

Culley, D ;

Yang, CP ;

Durst, R ;

Wrolstad, R .

JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 2005, 130 (02) :174-180

[3] Transcriptome analysis of an apple (Malus x domestica) yellow fruit somatic mutation identifies a gene network module highly associated with anthocyanin and epigenetic regulation [J].

El-Sharkawy, Islam ;

Liang, Dong ;

Xu, Kenong .

JOURNAL OF EXPERIMENTAL BOTANY, 2015, 66 (22) :7359-7376

[4] ABCC1, an ATP Binding Cassette Protein from Grape Berry, Transports Anthocyanidin 3-O-Glucosides [J].

Francisco, Rita Maria ;

Regalado, Ana ;

Ageorges, Agnes ;

Burla, Bo J. ;

Bassin, Barbara ;

Eisenach, Cornelia ;

Zarrouk, Olfa ;

Vialet, Sandrine ;

Marlin, Therese ;

Chaves, Maria Manuela ;

Martinoia, Enrico ;

Nagya, Reka .

PLANT CELL, 2013, 25 (05) :1840-1854

[5] Grapevine MATE-Type Proteins Act as Vacuolar H+-Dependent Acylated Anthocyanin Transporters [J].

Gomez, Camila ;

Terrier, Nancy ;

Torregrosa, Laurent ;

Vialet, Sandrine ;

Fournier-Level, Alexandre ;

Verries, Clotilde ;

Souquet, Jean-Marc ;

Mazauric, Jean-Paul ;

Klein, Markus ;

Cheynier, Veronique ;

Ageorges, Agnes .

PLANT PHYSIOLOGY, 2009, 150 (01) :402-415

[6] A multidrug resistance-associated protein involved in anthocyanin transport in Zea mays [J].

Goodman, CD ;

Casati, P ;

Walbot, V .

PLANT CELL, 2004, 16 (07) :1812-1826

[7] LcGST4 is an anthocyanin-related glutathione S-transferase gene in Litchi chinensis Sonn. [J].

Hu, Bing ;

Zhao, Jietang ;

Lai, Biao ;

Qin, Yonghua ;

Wang, Huicong ;

Hu, Guibing .

PLANT CELL REPORTS, 2016, 35 (04) :831-843

[8] MdMYB114 regulates anthocyanin biosynthesis and functions downstream of MdbZIP4-like in apple fruit [J].

Jiang, Shenghui ;

Sun, Qingguo ;

Zhang, Tianliang ;

Liu, Wenjun ;

Wang, Nan ;

Chen, Xuesen .

JOURNAL OF PLANT PHYSIOLOGY, 2021, 257

[9] TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis [J].

Kitamura, S ;

Shikazono, N ;

Tanaka, A .

PLANT JOURNAL, 2004, 37 (01) :104-114

[10] Molecular characterization of an anthocyanin-related glutathione S-transferase gene in cyclamen [J].

Kitamura, Satoshi ;

Akita, Yusuke ;

Ishizaka, Hiroshi ;

Narumi, Issay ;

Tanaka, Atsushi .

JOURNAL OF PLANT PHYSIOLOGY, 2012, 169 (06) :636-642

← 1 2 3 →