Effects and mechanisms of Zhizi Chuanxiong herb pair against atherosclerosis: an integration of network pharmacology, molecular docking, and experimental validation

被引:2
作者
Zhang, Yan [1 ]
Qi, Yifei [1 ]
Jia, Zijun [2 ]
Li, Yiming [1 ,3 ]
Wu, Liqi [1 ]
Zhou, Qingbing [1 ]
Xu, Fengqin [1 ]
机构
[1] China Acad Chinese Med Sci, Xiyuan Hosp, Inst Geriatr, Beijing, Peoples R China
[2] Comprehens Care China Japan Friendship Hosp, Beijing, Peoples R China
[3] Peking Univ, Dept Integrated Tradit & Western Med, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
Atherosclerosis; Macrophage polarization; Zhizi Chuanxiong herb pair; TNF/NF-kappa B axis; Network pharmacology; Molecular docking; ADHESION;
D O I
10.1186/s13020-023-00874-x
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Background The Zhizi Chuanxiong herb pair (ZCHP) can delay pathological progression of atherosclerosis (AS); however, its pharmacological mechanism remains unclear because of its complex components. The purpose of current study is to systematically investigate the anti-AS mechanism of ZCHP.Methods The databases of TCMSP, STITCH, SwissTargetPrediction, BATMAN-TCM, and ETCM were searched to predict the potential targets of ZCHP components. Disease targets associated with AS was retrieved from the GEO database. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analyses were executed using DAVID 6.8. Molecular docking method was employed to evaluate the core target binding to blood components, and animal experiments were performed to test action mechanism.Results A ZCHP-components-targets-AS network was constructed by using Cytoscape, included 11 main components and 52 candidate targets. Crucial genes were shown in the protein-protein interaction network, including TNF, IL-1 beta, IGF1, MMP9, COL1A1, CCR5, HMOX1, PTGS1, SELE, and SYK. KEGG enrichment illustrated that the NF-kappa B, Fc epsilon RI, and TNF signaling pathways were important for AS treatment. These results were validated by molecular docking. In ApoE(-/-) mice, ZCHP significantly reduced intima-media thickness, pulse wave velocity, plaque area, and serum lipid levels while increasing the difference between the end-diastolic and end-systolic diameters. Furthermore, ZCHP significantly decreased the mRNA and protein levels of TNF-alpha and IL-1 beta, suppressed NF-kappa B activation, and inhibited the M1 macrophage polarization marker CD86 in ApoE(-/-) mice.Conclusion This study combining network pharmacology, molecular biology, and animal experiments showed that ZCHP can alleviate AS by suppressing the TNF/NF-kappa B axis and M1 macrophage polarization.
引用
收藏
页数:12
相关论文
共 38 条
  • [11] Anti-atherosclerotic therapies: Milestones, challenges, and emerging innovations
    Hetherington, Isabella
    Totary-Jain, Hana
    [J]. MOLECULAR THERAPY, 2022, 30 (10) : 3106 - 3117
  • [12] TET1 Knockdown Inhibits Porphyromonas gingivalis LPS/IFN--Induced M1 Macrophage Polarization through the NF-B Pathway in THP-1 Cells
    Huang, Yanlan
    Tian, Cheng
    Li, Qimeng
    Xu, Qiong
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (08)
  • [13] Exosomal miR-30d-5p of neutrophils induces M1 macrophage polarization and primes macrophage pyroptosis in sepsis-related acute lung injury
    Jiao, Yang
    Zhang, Ti
    Zhang, Chengmi
    Ji, Haiying
    Tong, Xingyu
    Xia, Ran
    Wang, Wei
    Ma, Zhengliang
    Shi, Xueyin
    [J]. CRITICAL CARE, 2021, 25 (01)
  • [14] Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway
    Jin, Zheng
    Li, Junlong
    Pi, Jianbin
    Chu, Qingmin
    Wei, Weichao
    Du, Zhiyi
    Qing, Lijin
    Zhao, Xinjun
    Wu, Wei
    [J]. BIOMEDICINE & PHARMACOTHERAPY, 2020, 125
  • [15] Fully automated antibody structure prediction using BIOVIA tools: Validation study
    Kemmish, Helen
    Fasnacht, Marc
    Yan, Lisa
    [J]. PLOS ONE, 2017, 12 (05):
  • [16] Caffeic Acid Disturbs Monocyte Adhesion onto Cultured Endothelial Cells Stimulated by Adipokine Resistin
    Lee, Eun-Sook
    Park, Sin-Hye
    Kim, Min Soo
    Han, Seon-Young
    Kim, Hyun-Sung
    Kang, Young-Hee
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2012, 60 (10) : 2730 - 2739
  • [17] Crocin alleviates coronary atherosclerosis via inhibiting lipid synthesis and inducing M2 macrophage polarization
    Li, Jing
    Lei, Hong-tao
    Cao, Lei
    Mi, Yan-Ni
    Li, Sen
    Cao, Yong-Xiao
    [J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2018, 55 : 120 - 127
  • [18] Levistilide A reverses rat hepatic fibrosis by suppressing angiotensin II-induced hepatic stellate cells activation
    Li, Shu
    Zhao, Wei
    Zhao, Zhimin
    Cheng, Binbin
    Li, Shuang
    Liu, Chenghai
    [J]. MOLECULAR MEDICINE REPORTS, 2020, 22 (03) : 2191 - 2198
  • [19] Integrating Network Pharmacology and Experimental Validation to Decipher the Mechanism of Action of Huanglian Jiedu Decoction in Treating Atherosclerosis
    Liang, Jiahua
    Huang, Yingjie
    Mai, Zhexing
    Zhan, Qunzhang
    Lin, Hengchen
    Xie, Yuxin
    Wang, Haihao
    Liu, Yan
    Luo, Chuanjin
    [J]. DRUG DESIGN DEVELOPMENT AND THERAPY, 2021, 15 : 1779 - 1795
  • [20] CB-Dock: a web server for cavity detection-guided protein-ligand blind docking
    Liu, Yang
    Grimm, Maximilian
    Dai, Wen-tao
    Hou, Mu-chun
    Xiao, Zhi-Xiong
    Cao, Yang
    [J]. ACTA PHARMACOLOGICA SINICA, 2020, 41 (01) : 138 - 144