Subcellular RNA distribution and its change during human embryonic stem cell differentiation

被引：3

作者：

Zhou, Fanqi ^{[1
]}

Tan, Puwen ^{[2
]}

Liu, Siqi ^{[1
]}

Chang, Le ^{[1
]}

Yang, Jiabin ^{[1
]}

Sun, Mengyao ^{[1
]}

Guo, Yuehong ^{[1
]}

Si, Yanmin ^{[1
]}

Wang, Dong ^{[2
]}

Yu, Jia ^{[1
,3
,4
]}

Ma, Yanni ^{[1
,3
]}

机构：

[1] Chinese Acad Med Sci, Peking Union Med Coll, Inst Basic Med Sci, Key Lab RNA & Hematopoiet Regulat,State Key Lab Co, Beijing, Peoples R China

[2] Southern Med Univ, Sch Basic Med Sci, Dept Bioinformat, Guangzhou, Peoples R China

[3] Chinese Acad Med Sci, Inst Blood Transfus, Chengdu, Sichuan, Peoples R China

[4] Chinese Acad Med Sci & Peking Union Med Coll, Inst Hematol & Blood Dis Hosp, State Key Lab Expt Hematol, Natl Clin Res Ctr Blood Dis, Tianjin, Peoples R China

来源：

STEM CELL REPORTS | 2024年 / 19卷 / 01期

基金：

中国国家自然科学基金;

关键词：

MESSENGER-RNA; LOCALIZATION; PLURIPOTENCY; MECHANISMS; REVEALS;

D O I：

10.1016/j.stemcr.2023.11.007

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

The spatial localization of RNA within cells is closely related to its function and also involved in cell fate determination. However, the atlas of RNA distribution within cells and dynamic changes during the developmental process are largely unknown. In this study, five subcellular components, including cytoplasmic extract, membrane extract, soluble nuclear extract, chromatin -bound nuclear extract, and cytoskeletal extract, were isolated and the rules of subcellular RNA distribution in human embryonic stem cells (hESCs) and its change during hESC differentiation are summarized for the first time. The overall distribution patterns of coding and non -coding RNAs are revealed. Interestingly, some developmental genes are found to be transcribed but confined to the chromatin in undifferentiated hESC. Unexpectedly, alternative splicing and polyadenylation endow spatial heterogeneity among different isoforms of the same gene. Finally, the dynamic pattern of RNA distribution during hESC differentiation is characterized, which provides new clues for a comprehensive understanding hESC pluripotency and differentiation.

引用

页码：126 / 140

页数：15

共 45 条

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