Purification and antimicrobial mechanism of a novel bacteriocin produced by Lactiplantibacillus plantarum FB-2

Lactiplantibacillus plantarum, a major producer of bacteriocins, is generally considered a safe microorganism. In this study, Plantaricin FB-2 was extracted from L. plantarum FB-2 and studied for antimicrobial mechanism against Staphylococcus aureus. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed that the size of Plantaricin FB-2 is 1-3.3 kDa. Plantaricin FB-2 is non-hemolytic and its minimum inhibitory concentration against S. aureus ATCC6538 is about 256 & mu;g/mL. Plantaricin FB-2 exerts the antibacterial effect by destroying the structure of the bacterial cell membrane, increasing cell permeability, promoting the leakage of cytoplasmic contents (proteins, DNA, K+, Ca2+, etc.), and increasing intracellular reactive oxygen species levels. This study provides the theoretical basis for the development of L. plantarum produced Plantaricin FB-2 as a new antibacterial agent.