Evaluation of the Xpert Xpress GBS test for rapid detection of group B Streptococcus in pregnant women

被引：3

作者：

Wang, Feiling ^{[1
]}

Yi, Lehui ^{[1
,2
]}

Ming, Fang ^{[3
]}

Dong, Rui ^{[1
]}

Wang, Feng ^{[1
]}

Chen, Ruirui ^{[3
]}

Hu, Xiaoling ^{[4
]}

Chen, Xuri ^{[3
]}

Sun, Bo ^{[3
]}

Tang, Yi-Wei ^{[5
,6
]}

Zhu, Yuanfang ^{[3
]}

Wu, Lijuan ^{[1
,3
]}

机构：

[1] Shenzhen Baoan Womens & Childrens Hosp, Clin Lab, Shenzhen, Guangdong, Peoples R China

[2] Zunyi Med Univ, Dept Grad & Sci Res, Zhuhai Campus, Zhuhai, Guangdong, Peoples R China

[3] Shenzhen Baoan Womens & Childrens Hosp, Maternal Fetal Med Inst, Dept Obstet, Shenzhen Key Med Discipline, Shenzhen, Guangdong, Peoples R China

[4] Cepheid, Clin Affairs, Sunnyvale, CA USA

[5] Cepheid, Med Affairs, Sunnyvale, CA USA

[6] Danaher Diagnost Platform China, Shanghai, Peoples R China

来源：

MICROBIOLOGY SPECTRUM | 2024年 / 12卷 / 01期

基金：

中国国家自然科学基金;

关键词：

group B Streptococcus; Xpert Xpress GBS; enrichment culture; qPCR; direct culture; MATERNAL COLONIZATION; DISEASE;

D O I：

10.1128/spectrum.02206-23

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Group B Streptococcus (GBS) remains the leading cause of serious neonatal infection. Prenatal screening for vaginal and rectal GBS colonization is necessary in late pregnancy. Our objective was to compare the performance of four methods including the Xpert Xpress GBS, standard broth enrichment culture, real-time quantitative PCR (qPCR), and direct culture, for detecting of GBS in rectovaginal specimens. Among 939 pregnant women at 35-37 weeks of gestation recruited in the study, 168 (17.9%) were determined to be GBS-colonized by broth enrichment culture, the gold standard. The sensitivity and specificity of the Xpert Xpress GBS were 94.6% and 95.9%, respectively. The hands-on time and running time of the Xpert Xpress GBS were 1 minute and less than 1 hour, respectively. As a random-access, integrated device, the Xpert Xpress GBS test efficiently and accurately screens rectovaginal swabs for GBS colonization in pregnant women.

引用

页数：14

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