Nucleic Acids Isolation for Molecular Diagnostics: Present and Future of the Silica-based DNA/RNA Purification Technologies

被引：6

作者：

Mirna Lorena, Sanchez ^{[1
]}

Coronas Cynthia, Penaloza ^{[2
,3
]}

Maria Isabela, Avila Rodriguez ^{[4
]}

Mendieta Pamela, Viana ^{[4
]}

Hernandez Gabriela, Rivera ^{[2
,4
]}

Jorge, Benavides ^{[4
]}

Mariano, Grasselli ^{[1
]}

机构：

[1] Univ Nacl Quilmes, Dept Ciencia & Tecnol, Lab Mat Biotecnol LaMaBio, IMBICE, Bernal, Argentina

[2] Univ Nacl Quilmes, Dept Ciencia & Tecnol, Bernal, Argentina

[3] Dept Biol Mol, Lab Patol Quirurg & Citol Puebla, Puebla, Mexico

[4] Inst Invest Obesidad, Tecnol Monterrey, Monterrey, Mexico

来源：

SEPARATION AND PURIFICATION REVIEWS | 2023年 / 52卷 / 03期

关键词：

Silica; purification; nucleic-acids; diagnostics; A-CHIP PLATFORM; MAGNETIC BEADS; PIPETTE-TIP; SPIN-COLUMN; DNA; EXTRACTION; RNA; SIMULATION; SEPARATION; POLYMER;

D O I：

10.1080/15422119.2022.2053159

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

At present, most commercial kits for nucleic acid isolation are silica-based. In this regard, new strategies are under development with the aim of optimizing nucleic acid purification silica-based protocols. This review aims to summarize the evolution of such strategies and highlight the current necessities to be solved by new purification methods based on silica materials. In addition, it will perform a survey of the purified DNA/RNA quality and quantity for a subsequent technique. This information is of vital importance to consider for the success of molecular diagnostics. The text was designed to guide scientists and engineers in choosing suitable DNA/RNA purification techniques for research or bioprocessing applications.

引用

页码：193 / 204

页数：12

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