Vitamin D (VD3) Intensifies the Effects of Exercise and Prevents Alterations of Behavior, Brain Oxidative Stress, and Neuroinflammation, in Hemiparkinsonian Rats

被引：12
作者：
da Costa, Roberta Oliveira [1 ]
Jatai Gadelha-Filho, Carlos Vinicius [2 ]
Alexandre de Aquino, Pedro Everson [2 ]
Rodrigues Lima, Ludmila Araujo [1 ]
de Lucena, Jalles Dantas [1 ]
Correia Ribeiro, Wesley Lyeverton [2 ]
Viana Lima, Francisco Arnaldo [2 ]
Tavares Neves, Kelly Rose [2 ]
de Barros Viana, Glauce Socorro [1 ,2 ]
机构：
[1] Univ Fed Ceara, Fac Med, Grad Program Morphofunct Sci, Fortaleza, Ceara, Brazil
[2] Univ Fed Ceara, Fac Med, Grad Program Pharmacol, Fortaleza, Ceara, Brazil
来源：
NEUROCHEMICAL RESEARCH | 2023年 / 48卷 / 01期
关键词：
Parkinson's disease; Vitamin D3; Neuroinflammation; Oxidative stress; Exercise; Behavior; TYROSINE-HYDROXYLASE; PARKINSONS-DISEASE; DOPAMINE TRANSPORTER; D-RECEPTOR; FALLS; EXPRESSION; GLUTATHIONE; DEFICITS; GENE;
D O I：
10.1007/s11064-022-03728-4
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the present study, we investigated the effects of physical exercise in the presence of Vitamin D3 (VD3), on 6-hydroxydopamine (6-OHDA)-lesioned hemiparkinsonian rats. The animals were divided into sham-operated (SO), 6-OHDA-lesioned, and 6-OHDA-lesioned plus VD3 (1 mu g/kg, 21 days), in the absence (no exercise, NE) and presence (with exercise, WE) of physical exercise on a treadmill (30 min, speed of 20 cm/s, once a day/21 days). This procedure started, 24 h after the stereotaxic surgery (injections of 6-OHDA into the right striatum). The animals were then subjected to behavioral (rotarod, open field, and apomorphine tests) and their brain areas were dissected for neurochemical, dopamine (DA), 3,4-dihydroxyphenylacetic acid (DOPAC) determinations, and immunohistochemical studies for tyrosine hydroxylase (TH), dopamine transporter (DAT), and vitamin D receptor (VD3R). The effects on the brain oxidative stress: nitrite/nitrate, glutathione (GSH), and malondialdehyde (MDA) measurements were also evaluated. Behavioral changes of the 6-OHDA lesioned group were improved by exercise plus VD3. Similar results were observed in dopamine (DA) and 3,4-dihydroxyphenylacetic acid (DOPAC) concentrations increased by exercise and VD3, compared with SO groups. Additionally, tyrosine hydroxylase (TH) and dopamine transporter (DAT) immunoexpressions were decreased in the 6-OHDA-lesioned groups, with values normalized after exercise and VD3. The VD3 receptor immunoexpression decreased in the 6-OHDA (NE) group, and this was attenuated by exercise, especially after VD3. While 6-OHDA lesions increased, VD3 supplementation decreased the oxidative stress, which was intensified by exercise. VD3 showed neuroprotective properties that were intensified by physical exercise. These VD3 actions on hemiparkinsonian rats are possibly related to its antioxidant and anti-inflammatory effects.
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收藏
页码：142 / 160
页数：19
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