Stachyose with effect on anti-angiogenic activity from Salvia yunnanensis

被引:2
作者
Xu, Yangyang [1 ,2 ]
He, Fei [3 ]
Jin, Can [2 ]
Su, Juan [3 ]
Ding, Kan [2 ,3 ]
机构
[1] Zhongshan Hosp Tradit Chinese Med, Pharmacol Lab, Zhongshan 528401, Guangdong, Peoples R China
[2] Chinese Acad Sci, Zhongshan Inst Drug Discovery, Zhongshan 528400, Peoples R China
[3] Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, Glycochemistry & Glycobiol Lab, 555 Zu Chong Zhi Rd, Shanghai 201203, Peoples R China
基金
中国国家自然科学基金;
关键词
Salvia yunnanensis; Stachyose; Anti-angiogenic activity; Galectin-3; STRUCTURAL-CHARACTERIZATION; CANCER-CELL; PECTIC OLIGOSACCHARIDE; GALECTIN-3; DITERPENOIDS; OPTIMIZATION; ELUCIDATION; DERIVATIVES; METASTASIS; MODULATION;
D O I
10.1016/j.jff.2023.105971
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Salvia yunnanensis is a traditional Chinese medicine for promoting blood circulation and eliminating blood stasis. Previous researches reported that Tanshinone IIA from Salvia yunnanensis had anti-angiogenic effect, however, whether its carbohydrates with similar effect is unknown. Here, we showed that a stachyose (Sta) from Salvia yunnanensis was purified by DEAE SepharoseTM Fast Flow anion-exchange chromatography and Bio-Gel P-2 gel permeation chromatography. Structure analyses suggested that Sta was tetrasaccharide as alpha-Gal-(1 -> 6)-alpha-Gal- (1 -> 6)-alpha-Glc-(1 -> 2)-beta-Fru. Further sulfated Sta was made and the degree of sulfate substitution was measured as 0.98. Bioactivity studies showed that Sta might block human microvascular endothelial cells tube formation in vitro and in vivo without significant cytotoxicity. Mechanism study suggested that Sta might bind to Galectin-3 (KD: 5.777E- 6 ) and inhibit its expression to block angiogenesis linked to VEGFR/FAK and PDGFR/AKT/ERK signaling. Evidence also supported that Sta might be a potential compound for developing stachyose as an antiangiogenesis functional food.
引用
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页数:10
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