Single-cell sequencing reveals the immune landscape of breast cancer patients with brain metastasis

被引:3
作者
Zhou, Huaping [1 ]
He, Xiang [2 ]
Huang, Jia [3 ]
Zhong, Yumin [4 ]
Zhang, Leyao [1 ]
Ao, Xiang [1 ]
Zhao, Hailin [1 ]
Hu, Su [1 ]
Li, Hongsheng [1 ]
Huang, Jianqing [1 ,3 ]
Huang, Hongxin [1 ]
Liang, Hongling [1 ]
机构
[1] Guangzhou Med Univ, Affiliated Canc Hosp & Inst, Guangzhou, Peoples R China
[2] Cent South Univ, Xiangya Hosp, Key Lab Mol Radiat Oncol Hunan Prov, Changsha, Peoples R China
[3] Guangzhou Med Univ, Sch Hlth Management, Guangzhou, Peoples R China
[4] South China Univ Technol, Guangdong Prov Peoples Hosp, Guangdong Prov Key Lab Translat Med Lung Canc, Guangdong Lung Canc Inst,Guangdong Acad Med Sci,Sc, Guangzhou, Peoples R China
关键词
brain metastases; breast cancer; cerebrospinal fluid; single-cell RNA sequencing; tumor immune microenvironment; MICROENVIRONMENTAL LANDSCAPE; DENDRITIC CELLS; MACROPHAGES; RECEPTOR; TUMORS;
D O I
10.1111/1759-7714.15243
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Breast cancer has the highest incidence rate of cancer worldwide, and brain metastases (BrM) are among the most malignant cases. While some patients have benefited from immune checkpoint inhibitors (ICIs), the complex anatomical structure of the brain and the heterogeneity of metastatic tumors have made it difficult to characterize the tumor immune microenvironment (TME) of metastatic tumors. Methods: To address this, we used single-cell RNA sequencing (scRNA-seq) to analyze immune cells in the cerebrospinal fluid (CSF) of BrM patients with breast cancer, thereby providing a comprehensive view of the immune microenvironment landscape of BrM. Results: Based on canonical marker genes, we identified nine cell types, and further identified their subtypes through differential expression gene (DEG) analysis. We compared the changes in cells and functions in the immune microenvironment of patients with different prognoses. Our analysis revealed a series of genes that promote tumor immune function (CCR5, LYZ, IGKC, MS4A1, etc.) and inhibit tumor immune function (SCGB2A2, CD24, etc.). Conclusions: The scRNA-seq in CSF provides a noninvasive method to describe the TME of breast cancer patients and guide immunotherapy.
引用
收藏
页码:702 / 714
页数:13
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