A colorimetric aptasensor for detecting ochratoxin A based on label-free aptamer and gold nanozyme

被引：3

作者：

Tang, Jidong ^{[1
]}

Tian, Bing ^{[1
]}

Tao, Xiaoqi ^{[1
,2
]}

机构：

[1] Southwest Univ, Coll Food Sci, Chongqing 400715, Peoples R China

[2] Chongqing Key Lab Special Food Cobuilt Sichuan & C, Chongqing 400715, Peoples R China

来源：

ANALYTICAL SCIENCES | 2023年 / 39卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Gold nanoparticles; Ochratoxin A; Peroxidase-like activity; Aptasensor; NANOPARTICLES; ADSORPTION; BINDING; DNA;

D O I：

10.1007/s44211-023-00404-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, the aptamer of ochratoxin A (OTA) increased the negative charge density on the surface of gold nanoparticles (AuNPs) and promoted the release of hydroxyl radicals and Au3+ to enhance the peroxidase-like activity of the AuNPs. The OTA bound only to the aptamer and did not adsorb non-specifically to the AuNPs. Based on these two conclusions, a label-free colorimetric aptasensor was successfully developed, enabling the precise detection of OTA within the concentration range of 10-600 nM, with a remarkably low detection limit of 6.20 nM. The colorimetric aptasensor was applied to detect OTA in oats, corn, soybeans, rice, and glutinous rice.

引用

页码：1623 / 1626

页数：4

共 20 条

[1] Targeting G-quadruplexes in gene promoters: a novel anticancer strategy? [J].

Balasubramanian, Shankar ;

Hurley, Laurence H. ;

Neidle, Stephen .

NATURE REVIEWS DRUG DISCOVERY, 2011, 10 (04) :261-275

[2] Enhancement of the peroxidase-like activity of aptamers modified gold nanoclusters by bacteria for colorimetric detection of Salmonella typhimurium [J].

Chen, Qingmei ;

Gao, Ran ;

Jia, Li .

TALANTA, 2021, 221

[3] Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase [J].

Hizir, Mustafa Salih ;

Top, Meryem ;

Balcioglu, Mustafa ;

Rana, Muhit ;

Robertson, Neil M. ;

Shen, Fusheng ;

Sheng, Jia ;

Yigit, Mehmet V. .

ANALYTICAL CHEMISTRY, 2016, 88 (01) :600-605

[4]

Hu JT, 2015, ANALYST, V140, P3581, DOI [10.1039/c5an00107b, 10.1039/C5AN00107B]

[5] Thiazole Orange-Modified Carbon Dots for Ratiometric Fluorescence Detection of G-Quadruplex and Double-Stranded DNA [J].

Jin, Ming ;

Liu, Xiangjun ;

Zhang, Xin ;

Wang, Linlin ;

Bing, Tao ;

Zhang, Nan ;

Zhang, Yun ;

Shangguan, Dihua .

ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (30) :25166-25173

[6] Adsorption of DNA onto gold nanoparticles and graphene oxide: surface science and applications [J].

Liu, Juewen .

PHYSICAL CHEMISTRY CHEMICAL PHYSICS, 2012, 14 (30) :10485-10496

[7] Dopamine and Melamine Binding to Gold Nanoparticles Dominates Their Aptamer-Based Label-Free Colorimetric Sensing [J].

Liu, Xixia ;

He, Fan ;

Zhang, Fang ;

Zhang, Zijie ;

Huang, Zhicheng ;

Liu, Juewen .

ANALYTICAL CHEMISTRY, 2020, 92 (13) :9370-9378

[8] Specific High-Affinity Binding of Thiazole Orange to Triplex and G-Quadruplex DNA [J].

Lubitz, Irit ;

Zikich, Dragoslav ;

Kotlyar, Alexander .

BIOCHEMISTRY, 2010, 49 (17) :3567-3574

[9] Aptamer-mediated 'turn-off/turn-on' nanozyme activity of gold nanoparticles for kanamycin detection [J].

Sharma, Tarun Kumar ;

Ramanathan, Rajesh ;

Weerathunge, Pabudi ;

Mohammadtaheri, Mahsa ;

Daima, Hemant Kumar ;

Shukla, Ravi ;

Bansal, Vipul .

CHEMICAL COMMUNICATIONS, 2014, 50 (100) :15856-15859

[10] Colorimetric zearalenone assay based on the use of an aptamer and of gold nanoparticles with peroxidase-like activity [J].

Sun, Shumin ;

Zhao, Ran ;

Feng, Sumin ;

Xie, Yanli .

MICROCHIMICA ACTA, 2018, 185 (12)

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