Transcription-translation coupling: Recent advances and future perspectives

被引：10

作者：

Woodgate, Jason ^{[1
,2
]}

Zenkin, Nikolay ^{[1
]}

机构：

[1] Newcastle Univ, Biosci Inst, Fac Med Sci, Ctr Bacterial Cell Biol, Newcastle Upon Tyne, England

[2] Newcastle Univ, Biosci Inst, Fac Med Sci, Ctr Bacterial Cell Biol, Baddiley Clark Bldg,Richardson Rd, Newcastle Upon Tyne NE2 4AX, England

来源：

MOLECULAR MICROBIOLOGY | 2023年 / 120卷 / 04期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

bacterial gene expression; coupling of transcription and translation; CryoEM; expressome; molecular machines; transcription; translation; RNA CHAIN ELONGATION; TERMINATION FACTOR; STRUCTURAL BASIS; POLYMERASE; EXPRESSION; RHO; ARCHITECTURE; ATTENUATION; MECHANISM; FIDELITY;

D O I：

10.1111/mmi.15076

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The flow of genetic information from the chromosome to protein in all living organisms consists of two steps: (1) copying information coded in DNA into an mRNA intermediate via transcription by RNA polymerase, followed by (2) translation of this mRNA into a polypeptide by the ribosome. Unlike eukaryotes, where transcription and translation are separated by a nuclear envelope, in bacterial cells, these two processes occur within the same compartment. This means that a pioneering ribosome starts translation on nascent mRNA that is still being actively transcribed by RNA polymerase. This tethering via mRNA is referred to as 'coupling' of transcription and translation (CTT). CTT raises many questions regarding physical interactions and potential mutual regulation between these large (ribosome is similar to 2.5 MDa and RNA polymerase is 0.5 MDa) and powerful molecular machines. Accordingly, we will discuss some recently discovered structural and functional aspects of CTT.

引用

页码：539 / 546

页数：8

共 50 条

[1] Structural Basis of Transcription: RNA Polymerase Backtracking and Its Reactivation [J].