Effect of lanthanides on oxidation of Mn2+cations via a high-affinity Mn-binding site in photosystem II membranes

被引:0
作者
Lovyagina, E. R. [1 ]
Luneva, O. G. [1 ]
Loktyushkin, A. V. [1 ]
Semin, B. K. [1 ]
机构
[1] Lomonosov Moscow State Univ, Fac Biol, Dept Biophys, Moscow 119234, Russia
关键词
Photosystem II; Lanthanides; High -affinity Mn-binding site; Mn 2+oxidation; Oxygen -evolving complex; Calcium; OXYGEN-EVOLVING COMPLEX; ELECTRON DONATION; OXIDIZING SIDE; COLORIMETRIC DETERMINATION; CA2+-DEPLETED PSII; MANGANESE; CALCIUM; EVOLUTION; CA2+; PHOTOACTIVATION;
D O I
10.1016/j.jinorgbio.2023.112237
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Lanthanide cations (La3+ and Tb3+) bind to the Ca-binding site of the oxygen-evolving complex in Ca-depleted PSII membranes and irreversibly inhibit the oxygen evolution. On the other hand, EPR measurement of Mn2+ concentration in buffer revealed that lanthanide cations inhibit the light-dependent oxidation of Mn2+ cations via the high-affinity Mn-binding site in Mn-depleted PSII membranes, which suggests that they bind to and inhibit the high-affinity Mn-binding site of the oxygen-evolving complex. The inhibition is irreversible, bound Ln3+ cation could not be washed out from the sample. Calcium ion inhibits oxidation of Mn2+ (5 mu M) at very high concentration (tens mM) and the inhibition is reversible. In this work we measured the reduction rate of exogenic electron acceptor 2,6-dichlorophenolindophenol during the oxidation of Mn2+ cations in the Ca-depleted PSII and in the Ca-depleted PSII treated with lanthanides after extraction of Mn cluster from these preparations. We found that irreversible binding of the lanthanide cation to the Ca-binding site in the Ca-depleted PSII membranes leads to a partial inhibition of the high-affinity Mn-binding site.
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页数:8
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