Plasmin-Induced Lens Epithelial Cells Detachment for the Reduction of Posterior Capsular Opacification

被引:1
作者
Bai, Xiaomei [1 ,2 ]
Liang, Jingli [1 ,2 ]
Yin, Yufan [1 ,2 ]
Jiang, Yuanfeng [1 ,2 ]
Zhao, Fangyu [1 ,2 ]
Tian, Fang [1 ,2 ]
Chen, Xiteng [1 ,2 ]
Dong, Lijie [1 ,2 ,3 ]
Bu, Shaochong [1 ,2 ,3 ]
机构
[1] Tianjin Med Univ Eye Hosp, Natl Clin Res Ctr Ocular Dis, Tianjin Branch, Tianjin Key Lab Retinal Funct & Dis,Eye Inst, Tianjin, Peoples R China
[2] Tianjin Med Univ Eye Hosp, Sch Optometry, Tianjin, Peoples R China
[3] 251 Fukang Rd, Tianjin 300384, Peoples R China
来源
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY | 2023年 / 12卷 / 04期
基金
中国国家自然科学基金;
关键词
plasmin; posterior capsular opacification; fibrotic; cell detachment; PHARMACOLOGICAL VITREOLYSIS; ACTIVATION; FIBRINOLYSIS; FIBRONECTIN; PREVENTION; LAMININ; PCO;
D O I
10.1167/tvst.12.4.23
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
Purpose: We sought to evaluate the efficacy and safety of plasmin injection in the capsu-lar bag during the cataract operation for the prevention of posterior capsule opacifica-tion.Methods: Thirty-seven anterior capsular flaps taken from phacoemulsification surgery were immersed in either 1 & mu;g/mL plasmin (plasmin group, n = 27) or phosphate -buffered saline (control group, n =10) for 2 minutes and photographed after fixation and nuclear staining to compare the numbers of residual lens epithelial cells. In the animal experiments, the plasmin solution was injected into the capsular bag and remained for 5 minutes during hydrodissection or after lens extraction. The degree of posterior capsu-lar opacity of the rabbits at 2 months were photographed by slit lamp biomicroscopy. In HLE-B3 cell culture, the cell detachment rate, proliferation, and apoptosis after the plasmin digestion were analyzed.Results: The residual lens epithelial cell numbers on the capsule after plasmin treatment were 168 & PLUSMN; 190.7/mm2 in the 1 & mu;g/mL plasmin group, which was significantly lower than that of the control (1012 & PLUSMN; 798.8/mm2; P < 0.0001). In a rabbit model, the treatment of plasmin resulted in a significantly clearer posterior capsule compared with that of the control group at 2 months postoperatively.Conclusions: This study suggested that plasmin injection can induce effective lens epithelial cell detachment, which could be a promising adjunctive treatment to further improve the success rate in posterior capsule opacification prevention.Translational Relevance: Plasmin injection for lens epithelial cell detachment could significantly decrease the number of residual lens epithelial cells. This approach could be a promising treatment incorporating the current treatment approach to further improve the success rate in posterior capsule opacification prevention.
引用
收藏
页数:10
相关论文
共 35 条
  • [1] Apple DJ, 2020, OPHTHALMOLOGY, V127, pS29, DOI 10.1016/j.ophtha.2020.01.026
  • [2] Zinn's zonule
    Bassnett, Steven
    [J]. PROGRESS IN RETINAL AND EYE RESEARCH, 2021, 82
  • [3] Bhargava Rahul, 2015, J Ophthalmic Vis Res, V10, P37, DOI 10.4103/2008-322X.156101
  • [4] Collen D, 2001, Hematology Am Soc Hematol Educ Program, P1
  • [5] Water-mediated lysis of lens epithelial cells attached to lens capsule
    Crowston, JG
    Healey, PR
    Hopley, C
    Neilson, G
    Milverton, EJ
    Maloof, A
    [J]. JOURNAL OF CATARACT AND REFRACTIVE SURGERY, 2004, 30 (05) : 1102 - 1106
  • [6] The lens capsule
    Danysh, Brian P.
    Duncan, Melinda K.
    [J]. EXPERIMENTAL EYE RESEARCH, 2009, 88 (02) : 151 - 164
  • [7] Indocyanine Green Reduces the Viability of Human Lens Epithelial Cells and Promotes Cytolysis: An Ex Vivo Study
    He, Zi-Fang
    Zhao, Ying-Di
    Zhang, Si
    Chen, Fei-Fei
    Liu, Ya-Jun
    Zhang, Wen-Wen
    Xie, Zheng-Gao
    [J]. TRANSLATIONAL VISION SCIENCE & TECHNOLOGY, 2021, 10 (13):
  • [8] Functional hierarchy of plasminogen kringles 1 and 4 in fibrinolysis and plasmin-induced cell detachment and apoptosis
    Ho-Tin-Noé, B
    Rojas, G
    Vranckx, R
    Lijnen, HR
    Anglés-Cano, E
    [J]. FEBS JOURNAL, 2005, 272 (13) : 3387 - 3400
  • [9] Plasminogen activation-induced pericellular fibronectin proteolysis promotes fibroblast apoptosis
    Horowitz, Jeffrey C.
    Rogers, David S.
    Simon, Richard H.
    Sisson, Thomas H.
    Thannickal, Victor J.
    [J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2008, 38 (01) : 78 - 87
  • [10] Integrins: Bidirectional, allosteric signaling machines
    Hynes, RO
    [J]. CELL, 2002, 110 (06) : 673 - 687