Protein deuteration via algal amino acids to circumvent proton back-exchange for 1H-detected solid-state NMR

被引：0

作者：

Aucharova, Hanna ^{[1
]}

Klein, Alexander ^{[1
]}

Gomez, Sara Medina ^{[1
]}

Soeldner, Benedikt ^{[1
]}

Vasa, Suresh K. ^{[1
]}

Linser, Rasmus ^{[1
]}

机构：

[1] TU Dortmund Univ, Dept Chem & Chem Biol Biophys Chem, Otto Hahn Str 4a, D-44227 Dortmund, Germany

来源：

CHEMICAL COMMUNICATIONS | 2024年 / 60卷 / 22期

基金：

欧洲研究理事会;

关键词：

MEMBRANE-PROTEINS; SPECTROSCOPY;

D O I：

10.1039/d4cc00213j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

With perdeuteration, solid-state NMR spectroscopy of large proteins suffers from incomplete amide-proton back-exchange. Using a 72 kDa micro-crystalline protein, we show that deuteration exclusively via deuterated amino acids, well-established in solution to suppress sidechain protonation without proton back-exchange obstacles, provides spectral resolution comparable to perdeuterated preparations at intermediate spinning frequencies.

引用

收藏

页码：3083 / 3086

页数：4

相关论文

共 22 条

[1] High Resolution 1H-Detected Solid-State NMR Spectroscopy of Protein Aliphatic Resonances: Access to Tertiary Structure Information [J].

Asami, Sam ;

Schmieder, Peter ;

Reif, Bernd .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (43) :15133-15135

[2]

Gauto DF, 2022, NAT COMMUN, V13, DOI 10.1038/s41467-022-29423-0

[3]

Imbert L, 2021, METHODS MOL BIOL, V2199, P127, DOI 10.1007/978-1-0716-0892-0_8

[4] Atomic-resolution chemical characterization of (2x)72-kDa tryptophan synthase via four- and five-dimensional 1H-detected solid-state NMR [J].

Klein, Alexander ;

Rovo, Petra ;

Sakhrani, Varun V. ;

Wang, Yangyang ;

Holmes, Jacob B. ;

Liu, Viktoriia ;

Skowronek, Patricia ;

Kukuk, Laura ;

Vasa, Suresh K. ;

Guentert, Peter ;

Mueller, Leonard J. ;

Linser, Rasmus .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2022, 119 (04)

[5] 1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning [J].

Le Marchand, Tanguy ;

Schubeis, Tobias ;

Bonaccorsi, Marta ;

Paluch, Piotr ;

Lalli, Daniela ;

Pell, Andrew J. ;

Andreas, Loren B. ;

Jaudzems, Kristaps ;

Stanek, Jan ;

Pintacuda, Guido .

CHEMICAL REVIEWS, 2022, 122 (10) :9943-10018

[6] Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression [J].

Linser, Rasmus ;

Gelev, Vladimir ;

Hagn, Franz ;

Arthanari, Haribabu ;

Hyberts, Sven G. ;

Wagner, Gerhard .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2014, 136 (32) :11308-11310

[7] Proton-Detected Solid-State NMR Spectroscopy of Fibrillar and Membrane Proteins [J].

Linser, Rasmus ;

Dasari, Muralidhar ;

Hiller, Matthias ;

Higman, Victoria ;

Fink, Uwe ;

del Amo, Juan-Miguel Lopez ;

Markovic, Stefan ;

Handel, Liselotte ;

Kessler, Brigitte ;

Schmieder, Peter ;

Oesterhelt, Dieter ;

Oschkinat, Hartmut ;

Reif, Bernd .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2011, 50 (19) :4508-4512

[8] A strategy to obtain backbone resonance assignments of deuterated proteins in the presence of incomplete amide 2H/1H back-exchange [J].

Löhr, F ;

Katsemi, V ;

Hartleib, J ;

Günther, U ;

Rüterjans, H .

JOURNAL OF BIOMOLECULAR NMR, 2003, 25 (04) :291-311

[9]

Macek, 2024, J MAGN RESON, DOI [10.5194/mr-2024-1, DOI 10.5194/MR-2024-1]

[10] 1H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins InVitro and InSitu [J].

Medeiros-Silva, Joao ;

Mance, Deni ;

Daniels, Mark ;

Jekhmane, Shehrazade ;

Houben, Klaartje ;

Baldus, Marc ;

Weingarth, Markus .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 55 (43) :13606-13610