Characterization of a Bacillus velezensis strain as a potential biocontrol agent against soft rot of eggplant fruits

被引:18
作者
Zhang, Xiaoyun [1 ]
Xin, Yu [1 ]
Wang, Junyi [1 ]
Dhanasekaran, Solairaj [1 ]
Yue, Qingrong [1 ]
Feng, Faping [1 ]
Gu, Xiangyu [2 ]
Li, Bo [2 ]
Zhao, Lina [1 ]
Zhang, Hongyin [1 ]
机构
[1] Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Peoples R China
[2] Jiangsu Univ Sci & Technol, Sch Grain Sci & Technol, 2 Mengxi Rd, Zhenjiang 212003, Peoples R China
关键词
Antagonistic bacterium; Biological control; Inhibitory substance; Surfactin; ROS; CAROTOVORUM SUBSP CAROTOVORUM; PECTOBACTERIUM-CAROTOVORUM; LIPOPEPTIDES; RESISTANCE; SUBTILIS; RESPONSES;
D O I
10.1016/j.ijfoodmicro.2023.110480
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Postharvest soft rot of eggplant fruits caused by Pectobacterium carotovorum is a bacterial disease with a high disease incidence and produces substantial economic losses. This study aimed to control postharvest soft rot of eggplant fruits by Bacillus velezensis and investigate the possible control mechanisms based on the effects of B. velezensis on P. carotovorum subsp. carotovorum (Pcc) and eggplant fruits, respectively. B. velezensis effectively controlled postharvest soft rot of eggplant fruits and directly inhibited Pcc growth in vitro. The volatile metabolites produced by B. velezensis showed no inhibition on Pcc. Whereas the cell-free filtrate of B. velezensis significantly inhibited the growth of Pcc in vitro and in vivo. Notably, methanol-soluble precipitates obtained from cell-free filtrate showed significant inhibition on Pcc, and the primary inhibitory substances were identified as surfactin isoforms. Besides, iturin and fengycin isoforms with much lower relative abundance were also detected in the methanol-soluble precipitates. Furthermore, B. velezensis enhanced the activities of reactive oxygen species (ROS) scavenging enzymes in eggplant fruits that alleviated ROS and oxidative damage; thereby, B. velezensis enhanced the fruits' disease resistance.
引用
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页数:10
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