Pancreatic cancer cell line in responsive hydrogel microcapsules for drug evaluation

被引:6
作者
Song, Taiyu [1 ]
Zhang, Hui [2 ]
Liu, Guangling [3 ,6 ]
Qiu, Yudong [1 ,6 ]
Wang, Huan [1 ,4 ,5 ,6 ]
机构
[1] Nanjing Univ, Inst Translat Med, Dept Gen Surg, Affiliated Drum Tower Hosp,Med Sch, Nanjing, Peoples R China
[2] Southeast Univ, Sch Life Sci & Technol, Nanjing, Peoples R China
[3] Nanjing Univ, Dept Pediat, Med Sch, Affiliated Drum Tower Hosp, Nanjing, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 8, Dept Orthoped, Shenzhen, Peoples R China
[5] Shenzhen Univ, Sch Med, Dept Biomed Engn, Shenzhen, Peoples R China
[6] Nanjing Univ, Inst Translat Med, Dept Gen Surg, Affiliated Drum Tower Hosp,Med Sch, Nanjing 210002, Peoples R China
基金
中国国家自然科学基金;
关键词
composite hydrogel; drug evaluation; microcapsule; microfluidics; pancreatic cancer; MICROENVIRONMENT;
D O I
10.1002/VIW.20230048
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Drug therapies are the cornerstone of systemic treatment for pancreatic cancer patients. However, the relative outcome of drug evaluation is often hampered by the complex microenvironment of pancreatic cancer due to the lack of reasonable tumor models. Here, we proposed a novel platform that integrated pancreatic adenocarcinoma cells encapsulated into hydrogel microcapsules for three-dimensional (3D) tumor cultivation and antitumor agent evaluation. These hydrogel microcapsules contain alginate/poly (N-isopropyl acrylamide) (alginate/PNIPAM) shells and carboxymethyl cellulose cores, which are generated through the microfluidic electrospray technique. The microcapsules have the feature of rapid response to temperature, by which they can regulate the internal pressure environment. Besides, benefiting from good monodispersity, precise size control, and biocompatibility of these microcapsules, these wrapped tumor cells have the capacity for proliferating spontaneously and forming 3D tumor spheroids with good cell viability. We have demonstrated that pancreatic adenocarcinoma cells encapsulated in the composite microcapsules with different PNIPAM concentrations showed different drug sensitivity, which could be ascribed to the influence of external pressures environment. These results indicate that the tumor spheroids coated in these responsive microcapsules have great potential in the analysis of antitumor drug sensitivity. The outcome of drug evaluation is often hampered by the complex microenvironment of pancreatic cancer due to the lack of reasonable tumor models. The authors have proposed a novel platform that integrated pancreatic adenocarcinoma cells encapsulated into alginate/poly (N-isopropyl acrylamide)/Carboxymethyl cellulose hydrogel microcapsules for mincing external pressures environment of tumor cells and antitumor agent evaluation. The tumor spheroids coated in these responsive microcapsules have great potential in the analysis of antitumor drug sensitivity.image
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页数:9
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共 41 条
  • [21] Microenvironment drives cell state, plasticity, and drug response in pancreatic cancer
    Raghavan, Srivatsan
    Winter, Peter S.
    Navia, Andrew W.
    Williams, Hannah L.
    DenAdel, Alan
    Lowder, Kristen E.
    Galvez-Reyes, Jennyfer
    Kalekar, Radha L.
    Mulugeta, Nolawit
    Kapner, Kevin S.
    Raghavan, Manisha S.
    Borah, Ashir A.
    Liu, Nuo
    Vayrynen, Sara A.
    Costa, Andressa Dias
    Ng, Raymond W. S.
    Wang, Junning
    Hill, Emma K.
    Ragon, Dorisanne Y.
    Brais, Lauren K.
    Jaeger, Alex M.
    Spurr, Liam F.
    Li, Yvonne Y.
    Cherniack, Andrew D.
    Booker, Matthew A.
    Cohen, Elizabeth F.
    Tolstorukov, Michael Y.
    Wakiro, Isaac
    Rotem, Asaf
    Johnson, Bruce E.
    McFarland, James M.
    Sicinska, Ewa T.
    Jacks, Tyler E.
    Sullivan, Ryan J.
    Shapiro, Geoffrey, I
    Clancy, Thomas E.
    Perez, Kimberly
    Rubinson, Douglas A.
    Ng, Kimmie
    Cleary, James M.
    Crawford, Lorin
    Manalis, Scott R.
    Nowak, Jonathan A.
    Wolpin, Brian M.
    Hahn, William C.
    Aguirre, Andrew J.
    Shalek, Alex K.
    [J]. CELL, 2021, 184 (25) : 6119 - +
  • [22] Matrix stiffness induces epithelial-mesenchymal transition and promotes chemoresistance in pancreatic cancer cells
    Rice, A. J.
    Cortes, E.
    Lachowski, D.
    Cheung, B. C. H.
    Karim, S. A.
    Morton, J. P.
    Hernandez, A. del Rio
    [J]. ONCOGENESIS, 2017, 6 : e352 - e352
  • [23] Tumor matrix stiffness provides fertile soil for cancer stem cells
    Safaei, Sadegh
    Sajed, Roya
    Shariftabrizi, Ahmad
    Dorafshan, Shima
    Zanjani, Leili Saeednejad
    Manshadi, Masoumeh Dehghan
    Madjd, Zahra
    Ghods, Roya
    [J]. CANCER CELL INTERNATIONAL, 2023, 23 (01)
  • [24] In Situ Sprayed Difunctional Gel Avoiding Microenvironments Limitations to Treat Pressure Ulcers
    Shan, Jingyang
    Li, Xiuping
    Huang, Zhengzheng
    Kong, Bin
    Wang, Huan
    Ren, Lijie
    [J]. MACROMOLECULAR BIOSCIENCE, 2023, 23 (05)
  • [25] Chemotherapy for pancreatic cancer
    Springfeld, Christoph
    Jaeger, Dirk
    Buechler, Markus W.
    Strobel, Oliver
    Hackert, Thilo
    Palmer, Daniel H.
    Neoptolemos, John P.
    [J]. PRESSE MEDICALE, 2019, 48 (03): : E159 - E174
  • [26] MXene-Integrated Microneedle Patches with Innate Molecule Encapsulation for Wound Healing
    Sun, Lingyu
    Fan, Lu
    Bian, Feika
    Chen, Guopu
    Wang, Yuetong
    Zhao, Yuanjin
    [J]. RESEARCH, 2021, 2021
  • [27] Wang H., 2021, ENG REGEN, V3, P407
  • [28] Artificial cells from microfluidic electrospray for reactive oxygen species scavenging
    Wang, Huan
    Zhang, Hui
    Kong, Bin
    Wang, Peng
    Shen, Huiyong
    [J]. MATERIALS & DESIGN, 2023, 234
  • [29] Polymer-based responsive structural color materials
    Wang, Huan
    Zhang, Hui
    Chen, Zhuoyue
    Zhao, Yuanjin
    Gu, Zhuxiao
    Shang, Luoran
    [J]. PROGRESS IN MATERIALS SCIENCE, 2023, 135
  • [30] Responsive Janus Structural Color Hydrogel Micromotors for Label-Free Multiplex Assays
    Wang, Huan
    Cai, Lijun
    Zhang, Dagan
    Shang, Luoran
    Zhao, Yuanjin
    [J]. RESEARCH, 2021, 2021