Ranuncoside's attenuation of scopolamine-induced memory impairment in mice via Nrf2 and NF-KB signaling

被引:5
作者
Salahuddin, Hafiza Sara [1 ]
Attaullah, Sobia [1 ]
Shah, Shahid Ali [2 ,3 ]
Khan, SanaUllah [4 ]
Zahid, Muhammad [1 ]
Ullah, Mujeeb [1 ]
Khayyam
Salahuddin, Sidra [5 ]
Gul, Seema [1 ]
Alsugoor, Mahdi H. [6 ]
机构
[1] Islamia Coll, Dept Zool, Peshawar, Khyber Pakhtunk, Pakistan
[2] Neuro Mol Med Res Ctr NMMRC, Ring Rd, Peshawar, Kpk, Pakistan
[3] Univ Haripur, Kpk, Pakistan
[4] Univ Peshawar, Dept Zool, Khyber Pakhtunkhwa, Pakistan
[5] Hayatabad Med Complex, Peshawar, Khyber Pakhtunk, Pakistan
[6] Umm Al Qura Univ, Coll Hlth Sci AlQunfudah, Dept Emergency Med Serv, Mecca 21912, Saudi Arabia
关键词
Ranuncoside; Scopolamine; Memory impairment; Behavioral test; Alzheimer's disease; ALZHEIMERS-DISEASE; AMYLOID-BETA; HYPERGLYCEMIA; ANTIOXIDANT; INHIBITION;
D O I
10.1016/j.jsps.2023.101702
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Scopolamine is a well-known pharmacological agent responsible for causing memory impairment in animals, as well as oxidative stress and neuroinflammation inducer which lead to the development of Alzheimer disease. Although a cure for Alzheimer's disease is unavailable. Ranuncoside, a metabolite obtained from a medicinal plant has demonstrated antioxidant and anti-inflammatory properties in vitro, making it a promising treatment with potential anti-Alzheimer disease properties. However, as ranuncoside has not been evaluated for its antioxidant and anti-neuroinflammatory properties in any in vivo model, our study aimed to evaluate its neurotherapeutic efficacy against scopolamineinduced memory impairment in adult male albino mice. Mice were randomly divided into four experimental groups.Mice of group I was injected with saline, group II was injected with scopolamine (1 mg/kg/day) for 3 weeks. After receiving a daily injection of scopolamine for 1 week, the mice of group III were injected with ranuncoside (10 mg/kg) every other day for 2 weeks along with scopolamine daily and group IV were injected with ranuncoside on 5th alternate days. Behavioral tests (i.e., Morris water maze and Y-maze) were performed to determine the memory-enhancing effect of ranuncoside against scopolamine's memory deleterious effect. Western blot analysis was also performed to further elucidate the anti-neuroinflammatory and antioxidant effects of ranuncoside against scopolamine-induced neuroinflammation and oxidative stress.Our results showed memory-enhancing, anti-neuroinflammatory effect, and antioxidant effects of ranuncoside against scopolamine by increasing the expression of the endogenous antioxidant system (i.e., Nrf2 and HO-1), followed by blocking neuroinflammatory markers such as NF-KB, COX-2, and TNF-a. The results also revealed that ranuncoside possesses hypoglycemic and hypolipidemic effects against scopolamine-induced hyperglycemia and hyperlipidemia in mice as well as scopolamine's hyperglycemic effect. In conclusion, our findings suggest that ranuncoside could be a potential agent for the management of Alzheimer's disease, hyperglycemia, and hyperlipidemia.& COPY; 2023 The Authors. Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
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页数:10
相关论文
共 62 条
[51]   Multifarious roles of mTOR signaling in cognitive aging and cerebrovascular dysfunction of Alzheimer's disease [J].
Uddin, Md. Sahab ;
Rahman, Md. Ataur ;
Kabir, Md. Tanvir ;
Behl, Tapan ;
Mathew, Bijo ;
Perveen, Asma ;
Barreto, George E. ;
Bin-Jumah, May N. ;
Abdel-Daim, Mohamed M. ;
Ashraf, Ghulam Md .
IUBMB LIFE, 2020, 72 (09) :1843-1855
[52]   Vanillic acid attenuates Aβ1-42-induced oxidative stress and cognitive impairment in mice [J].
Ul Amin, Faiz ;
Shah, Shahid Ali ;
Kim, Myeong Ok .
SCIENTIFIC REPORTS, 2017, 7
[53]   Natural Antioxidant Anthocyanins-A Hidden Therapeutic Candidate in Metabolic Disorders with Major Focus in Neurodegeneration [J].
Ullah, Rahat ;
Khan, Mehtab ;
Shah, Shahid Ali ;
Saeed, Kamran ;
Kim, Myeong Ok .
NUTRIENTS, 2019, 11 (06)
[54]  
Uto A., 2021, Endocrinology, V162, pbqab193, DOI [10.1210/endocr/bqab193, DOI 10.1210/ENDOCR/BQAB193]
[55]   Mitochondria dysfunction in the pathogenesis of Alzheimer's disease: recent advances [J].
Wang, Wenzhang ;
Zhao, Fanpeng ;
Ma, Xiaopin ;
Perry, George ;
Zhu, Xiongwei .
MOLECULAR NEURODEGENERATION, 2020, 15 (01)
[56]   Neuroprotective Effects of Euonymus alatus Extract on Scopolamine-Induced Memory Deficits in Mice [J].
Woo, Yunju ;
Lim, Ji Sun ;
Oh, Jisun ;
Lee, Jeong Soon ;
Kim, Jong-Sang .
ANTIOXIDANTS, 2020, 9 (05)
[57]   Raspberry anthocyanin consumption prevents diet-induced obesity by alleviating oxidative stress and modulating hepatic lipid metabolism [J].
Wu, Tao ;
Yang, Lu ;
Guo, Xueqi ;
Zhang, Min ;
Liu, Rui ;
Sui, Wenjie .
FOOD & FUNCTION, 2018, 9 (04) :2112-2120
[58]   Catalpol Weakens Depressive-like Behavior in Mice with Streptozotocin-induced Hyperglycemia via PI3K/AKT/Nrf2/HO-1 Signaling Pathway [J].
Wu, Xiaohui ;
Wang, Junming ;
Song, Lingling ;
Guan, Yuechen ;
Cao, Can ;
Cui, Ying ;
Zhang, Yueyue ;
Liu, Chen .
NEUROSCIENCE, 2021, 473 :102-118
[59]  
Yadang F.S.A., 2020, Int J Alzheimers Dis, V2020, P1, DOI [10.1155/2020/6372059, DOI 10.1155/2020/6372059]
[60]   Amyloid Beta Peptides and Th1 Cytokines Modulate Human Brain Vascular Smooth Muscle Tonic Contractile Capacity In Vitro: Relevance to Alzheimer's Disease? [J].
Yun, J. Winny ;
Washington, Caretia ;
McCormick, Joi ;
Stevenson, Emily ;
Alexander, J. Steven .
PATHOPHYSIOLOGY, 2021, 28 (01) :64-75