Indole-3-carbinol (I3C) reduces apoptosis and improves neurological function after cerebral ischemia-reperfusion injury by modulating microglia inflammation

被引：9
作者：
Peng, Long [1 ,2 ]
Zhu, Xingjia [1 ]
Wang, Chenxing [1 ]
Jiang, Qiaoji [3 ]
Yu, Shian [4 ]
Song, Gaochao [5 ]
Liu, Qianqian [1 ]
Gong, Peipei [1 ]
机构：
[1] Nantong Univ, Med Sch, Dept Neurosurg, Affiliated Hosp, Nantong, Peoples R China
[2] Ganzhou Municipal Hosp, Guangdong Prov Peoples Hosp, Dept Neurosurg, Ganzhou Hosp, Ganzhou, Peoples R China
[3] Xuzhou Med Univ, Dept Neurosurg, Affiliated Yancheng Clin Coll, Yancheng 224000, Jiangsu Provinc, Peoples R China
[4] Nanchang Univ, Dept Surg, Infect Dis Hosp, Nanchang Hosp 9, Nanchang, Peoples R China
[5] Nanjing Med Univ, Dept Neurosurg, Affiliated Hosp 2, Nanjing, Peoples R China
来源：
SCIENTIFIC REPORTS | 2024年 / 14卷 / 01期
关键词：
D O I：
10.1038/s41598-024-53636-6
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Indole-3-carbinol(I3C) is a tumor chemopreventive substance that can be extracted from cruciferous vegetables. Indole-3-carbinol (I3C) has been shown to have antioxidant and anti-inflammatory effects. In this study, we investigated the cerebral protective effects of I3C in an in vivo rats model of middle cerebral artery occlusion (MCAO). 8-10 Week-Old male SD rat received I3C (150 mg/kg, once daily) for 3 days and underwent 3 h of middle cerebral artery occlusion (MCAO) followed by reperfusion. The results showed that I3C pretreatment (150 mg/kg, once daily) prevented CIRI-induced cerebral infarction in rats. I3C pretreatment also decreased the mRNA expression levels of several apoptotic proteins, including Bax, caspase-3 and caspase-9, by increasing the mRNA expression levels of the anti-apoptotic protein Bcl-2. Inhibited apoptosis in the brain cells of MCAO rats. In addition, we found that I3C pretreatment reduced neuronal loss, promoted neurological recovery after ischemia-reperfusion injury and increased seven-day survival in MCAO rats. I3C pretreatment also significantly reduced the expression of inducible nitric oxide synthase (INOS), interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) mRNA in ischemic brain tissue; Increased expression of interleukin-4 (IL-4) and interleukin-10 (IL-10) mRNA. At the same time, I3C pretreatment significantly decreased the expression of the M1 microglial marker IBA1 after cerebral ischemia-reperfusion injury and increased the expression of these results in the M2 microglial marker CD206. I3C pretreatment also significantly decreased apoptosis and death of HAPI microglial cells after hypoxia induction, decreased interleukin-1 beta (IL-1 beta) and interleukin-6 (IL-6) mRNA The expression of interleukin-4 (IL-4) and interleukin-10 (IL-10) mRNAs was increased. These results suggest that I3C protects the brain from CIRI by regulating the anti-inflammatory and anti-apoptotic effects of microglia.
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页数：13
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