Expression of Ice Nucleation Protein in Bacillus amyloliquefaciens and Its Application in Food Freezing Process

被引：2

作者：

Song, Rong ^{[1
,2
]}

Jiang, Cong ^{[1
,2
]}

Zhu, Jing ^{[3
]}

Liu, Jia ^{[4
]}

Zhang, Li ^{[5
]}

Zuo, Jingnan ^{[1
,2
]}

Zheng, Wei ^{[1
,2
]}

Liu, Shilin ^{[1
,2
]}

Huang, Qingrong ^{[5
]}

Wei, Xuetuan ^{[1
,2
]}

Chen, Yijie ^{[1
,2
]}

机构：

[1] Minist Educ, Key Lab Environm Correlat Dietol, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

[3] Hubei Inst Measurement & Testing Technol, Wuhan 430070, Peoples R China

[4] Yangtze Univ, Coll Life Sci, Jingzhou 434023, Peoples R China

[5] Rutgers State Univ, Dept Food Sci, New Brunswick, NJ 08901 USA

来源：

FOODS | 2023年 / 12卷 / 21期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

Bacillus amyloliquefaciens; frozen food; ice nucleation activity; ice nucleation proteins (INPs); recombinant protein expressing; ACTIVE BACTERIA; EFFICIENCY; QUALITY; GENE;

D O I：

10.3390/foods12213896

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To produce food-grade ice nucleators, a 3.77 kb ice nucleation gene (iceE) isolated from Pantoea agglomerans (Erwinia herbicola) was introduced into the Gram-positive microorganism Bacillus amyloliquefaciens for the first time. The differential scanning calorimetry (DSC) results indicated that recombined strain B9-INP was an effective ice nucleator for controlling the supercooling point of distilled water at low concentrations. In the presence of B9-INP cells, model food systems, including sucrose solution and sodium chloride solution, different pH solutions froze at a relatively high subzero temperature, thus increasing the supercooling point by 5.8 similar to 16.7 degrees C. Moreover, B9-INP also facilitated model and real food systems to freeze at -6 degrees C. This recombinant strain not only improved the freezing temperature of food systems but also shortened the total freezing time, thus saving energy and reducing consumption. The results suggest that B9-INP has great application potential in the frozen food industry.

引用

页数：15

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