Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion

被引：3

作者：

Meiers, Joscha ^{[1
,2
,3
]}

Dastbaz, Jan ^{[2
,4
,5
]}

Adam, Sebastian ^{[2
,6
]}

Rasheed, Sari ^{[2
,4
,5
]}

Kirsch, Susanne H. ^{[2
,5
]}

Meiser, Peter ^{[7
]}

Gross, Peter ^{[8
]}

Mueller, Rolf ^{[2
,4
,5
]}

Titz, Alexander ^{[1
,2
,3
]}

机构：

[1] Helmholtz Ctr Infect Res, Helmholtz Inst Pharmaceut Res Saarland HIPS, Chem Biol Carbohydrates CBCH, D-66123 Saarbrucken, Germany

[2] Deutsch Zentrum Far Infektionsforsch DZIF Standor, D-38124 Braunschweig, Germany

[3] Saarland Univ, Dept Chem, D-66123 Saarbrucken, Germany

[4] Saarland Univ, Dept Pharm, D-66123 Saarbrucken, Germany

[5] Helmholtz Ctr Infect Res, Helmholtz Inst Pharmaceut Res Saarland HIPS, Microbial Nat Prod MINS, D-66123 Saarbrucken, Germany

[6] Helmholtz Inst Pharmaceut Res Saarland HIPS, Helmholtz Ctr Infect Res, Drug Design & Optimisat DDOP, D-66123 Saarbracken, Germany

[7] URSAPHARM Arzneimittel GmbH, D-66129 Saarbrucken, Germany

[8] Hsch Kaiserslautern, Prot Chem Grp, D-66953 Pirmasens 66953, Germany

来源：

CHEMBIOCHEM | 2023年 / 24卷 / 03期

关键词：

bromelain; carbohydrates; lectin; mannose; SARS-CoV-2; GLYCAN ARRAY; ASSAY;

D O I：

10.1002/cbic.202200463

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar K-D in a carbohydrate-dependent fashion.

引用

页数：13

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