Pineapple Lectin AcmJRL Binds SARS-CoV-2 Spike Protein in a Carbohydrate-Dependent Fashion

被引：3
作者：
Meiers, Joscha [1 ,2 ,3 ]
Dastbaz, Jan [2 ,4 ,5 ]
Adam, Sebastian [2 ,6 ]
Rasheed, Sari [2 ,4 ,5 ]
Kirsch, Susanne H. [2 ,5 ]
Meiser, Peter [7 ]
Gross, Peter [8 ]
Mueller, Rolf [2 ,4 ,5 ]
Titz, Alexander [1 ,2 ,3 ]
机构：
[1] Helmholtz Ctr Infect Res, Helmholtz Inst Pharmaceut Res Saarland HIPS, Chem Biol Carbohydrates CBCH, D-66123 Saarbrucken, Germany
[2] Deutsch Zentrum Far Infektionsforsch DZIF Standor, D-38124 Braunschweig, Germany
[3] Saarland Univ, Dept Chem, D-66123 Saarbrucken, Germany
[4] Saarland Univ, Dept Pharm, D-66123 Saarbrucken, Germany
[5] Helmholtz Ctr Infect Res, Helmholtz Inst Pharmaceut Res Saarland HIPS, Microbial Nat Prod MINS, D-66123 Saarbrucken, Germany
[6] Helmholtz Inst Pharmaceut Res Saarland HIPS, Helmholtz Ctr Infect Res, Drug Design & Optimisat DDOP, D-66123 Saarbracken, Germany
[7] URSAPHARM Arzneimittel GmbH, D-66129 Saarbrucken, Germany
[8] Hsch Kaiserslautern, Prot Chem Grp, D-66953 Pirmasens 66953, Germany
来源：
CHEMBIOCHEM | 2023年 / 24卷 / 03期
关键词：
bromelain; carbohydrates; lectin; mannose; SARS-CoV-2; GLYCAN ARRAY; ASSAY;
D O I：
10.1002/cbic.202200463
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The highly glycosylated spike protein of SARS-CoV-2 is essential for infection and constitutes a prime target for antiviral agents and vaccines. The pineapple-derived jacalin-related lectin AcmJRL is present in the medication bromelain in significant quantities and has previously been described to bind mannosides. Here, we performed a large ligand screening of AcmJRL by glycan array analysis, quantified the interaction with carbohydrates and validated high-mannose glycans as preferred ligands. Because the SARS-CoV-2 spike protein was previously reported to carry a high proportion of high-mannose N-glycans, we tested the binding of AcmJRL to the recombinantly produced extraviral domain of spike protein. We could demonstrate that AcmJRL binds the spike protein with a low-micromolar K-D in a carbohydrate-dependent fashion.
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页数：13
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